DVOŘÁK, Petr, D. HORÁK, J. KROUPOVÁ and M. ŠLOUF. Poly(2-hydroxyethyl methacrylate)-based slabs as a mouse embryonic stem cell support. Biomaterials. 2004, vol. 25, No 22, p. 5249-5260. ISSN 0142-9612.
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Basic information
Original name Poly(2-hydroxyethyl methacrylate)-based slabs as a mouse embryonic stem cell support
Authors DVOŘÁK, Petr, D. HORÁK, J. KROUPOVÁ and M. ŠLOUF.
Edition Biomaterials, 2004, 0142-9612.
Other information
Original language English
Type of outcome Article in a journal
Field of Study Genetics and molecular biology
Country of publisher United Kingdom of Great Britain and Northern Ireland
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 3.799
Organization unit Faculty of Science
UT WoS 000221366800004
Keywords in English stem cell
Tags stem cell
Changed by Changed by: prof. Ing. Petr Dvořák, CSc., učo 47260. Changed: 1/2/2006 13:44.
Abstract
Poly(2-hydroxyethyl methacrylate) (PHEMA) crosslinked with ethylene dimethacrylate (EDMA) or N,O-dimethacryloylhydroxylamine (DMHA) was obtained in the form of slabs by bulk radical polymerization. Two porosity-inducing methods were investigated, phase separation using a low-molecular-weight porogen and a salt-leaching technique using NaCl and saccharose. Compared with the phase separation, the salt-leaching created open porous structures with voids of the size and shape of crystallites. To address its potentials in the context of stem cell therapies, undifferentiated mouse embryonic stem cells D3 (ES D3 cells) were seeded on the slabs and analyzed for the ability to grow on different types of non-degradable and/or degradable porous PHEMA hydrogels. The cells were able to proliferate only on PHEMA crosslinked with EDMA or 2 wt% DMHA. In order to assess the effect of gelatin, which is routinely used for ES cell cultures, PHEMA slabs were soaked in gelatin solutions and compared the number of cells on gelatin-treated and untreated slabs 4 days after cell seeding. Surprisingly, the number of cells was only slightly higher on gelatin-treated slabs.
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