J 2004

Poly(2-hydroxyethyl methacrylate)-based slabs as a mouse embryonic stem cell support

DVOŘÁK, Petr, D. HORÁK, J. KROUPOVÁ and M. ŠLOUF

Basic information

Original name

Poly(2-hydroxyethyl methacrylate)-based slabs as a mouse embryonic stem cell support

Authors

DVOŘÁK, Petr, D. HORÁK, J. KROUPOVÁ and M. ŠLOUF

Edition

Biomaterials, 2004, 0142-9612

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

Genetics and molecular biology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 3.799

Organization unit

Faculty of Science

UT WoS

000221366800004

Keywords in English

stem cell

Tags

Změněno: 1/2/2006 13:44, prof. Ing. Petr Dvořák, CSc.

Abstract

V originále

Poly(2-hydroxyethyl methacrylate) (PHEMA) crosslinked with ethylene dimethacrylate (EDMA) or N,O-dimethacryloylhydroxylamine (DMHA) was obtained in the form of slabs by bulk radical polymerization. Two porosity-inducing methods were investigated, phase separation using a low-molecular-weight porogen and a salt-leaching technique using NaCl and saccharose. Compared with the phase separation, the salt-leaching created open porous structures with voids of the size and shape of crystallites. To address its potentials in the context of stem cell therapies, undifferentiated mouse embryonic stem cells D3 (ES D3 cells) were seeded on the slabs and analyzed for the ability to grow on different types of non-degradable and/or degradable porous PHEMA hydrogels. The cells were able to proliferate only on PHEMA crosslinked with EDMA or 2 wt% DMHA. In order to assess the effect of gelatin, which is routinely used for ES cell cultures, PHEMA slabs were soaked in gelatin solutions and compared the number of cells on gelatin-treated and untreated slabs 4 days after cell seeding. Surprisingly, the number of cells was only slightly higher on gelatin-treated slabs.