DVOŘÁK, Petr, Aleš HAMPL and J. KOHOUTEK. Temporal distribution of CDK4, CDK6, D-type cyclins, and p27 in developing mouse oocytes. Biology of reproduction. 2004, vol. 70, No 1, p. 139-145. ISSN 0006-3363.
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Basic information
Original name Temporal distribution of CDK4, CDK6, D-type cyclins, and p27 in developing mouse oocytes
Authors DVOŘÁK, Petr, Aleš HAMPL and J. KOHOUTEK.
Edition Biology of reproduction, 2004, 0006-3363.
Other information
Original language English
Type of outcome Article in a journal
Field of Study Genetics and molecular biology
Country of publisher United States of America
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 3.550
Organization unit Faculty of Science
UT WoS 000187572500019
Keywords in English oocyte; cell
Tags cell, oocyte
Changed by Changed by: prof. Ing. Petr Dvořák, CSc., učo 47260. Changed: 1/2/2006 13:44.
Abstract
Various molecular interactions not operating in other cell types are most likely required for mammalian oocytes to develop into fully competent eggs. This study seeks to initiate analyses of the potential oocyte-specific functions of regulators of G1/S progression-CDK4, CDK6, D-type cyclins, and p27-by first determining their expression patterns in growing and maturing mouse oocytes and in mouse embryos early after fertilization. Western blot and immunofluorescence analyses on isolated oocytes were employed to evaluate both their levels and their localization. The data show that 1). mouse oocytes contain significant amounts of all studied regulators; 2). their amounts and localization undergo dramatic changes as the oocytes grow, meiotically mature, and transit into embryogenesis; and 3). some regulators (CDK4, CDK6, cyclin D2, and p27) appear in unusual, most likely posttranslationally modified, forms. These data distinguish G1/S regulators as the potential players in molecular processes that are important for oocytes to function normally.
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