BRYJA, V., J. PACHERNÍK, L. KUBALA, Aleš HAMPL and P. DVOŘÁK. The reverse tetracycline-controlled transactivator rtTA2s-S2 is toxic in mouse embryonic stem cells. Reproduction, nutrition, development. 2003, vol. 43, No 6, p. 477-486. ISSN 0926-5287.
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Basic information
Original name The reverse tetracycline-controlled transactivator rtTA2s-S2 is toxic in mouse embryonic stem cells
Authors BRYJA, V., J. PACHERNÍK, L. KUBALA, Aleš HAMPL and P. DVOŘÁK.
Edition Reproduction, nutrition, development, 2003, 0926-5287.
Other information
Original language English
Type of outcome Article in a journal
Field of Study Genetics and molecular biology
Country of publisher France
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 0.780
Organization unit Faculty of Science
Keywords in English stem cells
Tags stem cells
Changed by Changed by: prof. Mgr. Vítězslav Bryja, Ph.D., učo 11088. Changed: 29/4/2009 10:57.
Abstract
The efficient and reversible control of transgene expression is a powerful tool for the correct manipulation of embryonic stem cells in both cell therapy and transgenesis. The aim of this work was to investigate the possibilities of recently developed reverse tetracycline-controlled transactivator rtTA2s-S2. We show that the rtTA2s-S2 is useful for transient inducible expression of genes in embryonic stem cells. However, we found that it was not possible to establish mouse embryonic stem cell lines stably expressing this transactivator. Using the viral IRES sequence which couples the expression of rtTA2s-S2 and neomycin phosphotransferase, we found that embryonic stem cells expressing rtTA2s-S2 are not capable of growing in the presence of G418. Our results indicate that this transactivator is toxic to ES cells and raise the need for the development of other strategies for stable and inducible expression of genes in ES cells.
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