BRYJA, V., J. PACHERNÍK, L. KUBALA, Aleš HAMPL and P. DVOŘÁK. The reverse tetracycline-controlled transactivator rtTA2s-S2 is toxic in mouse embryonic stem cells. Reproduction, nutrition, development. 2003, vol. 43, No 6, p. 477-486. ISSN 0926-5287. |
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@article{617966, author = {Bryja, V. and Pacherník, J. and Kubala, L. and Hampl, Aleš and Dvořák, P.}, article_number = {6}, keywords = {stem cells}, language = {eng}, issn = {0926-5287}, journal = {Reproduction, nutrition, development}, title = {The reverse tetracycline-controlled transactivator rtTA2s-S2 is toxic in mouse embryonic stem cells}, volume = {43}, year = {2003} }
TY - JOUR ID - 617966 AU - Bryja, V. - Pacherník, J. - Kubala, L. - Hampl, Aleš - Dvořák, P. PY - 2003 TI - The reverse tetracycline-controlled transactivator rtTA2s-S2 is toxic in mouse embryonic stem cells JF - Reproduction, nutrition, development VL - 43 IS - 6 SP - 477-486 EP - 477-486 SN - 09265287 KW - stem cells N2 - The efficient and reversible control of transgene expression is a powerful tool for the correct manipulation of embryonic stem cells in both cell therapy and transgenesis. The aim of this work was to investigate the possibilities of recently developed reverse tetracycline-controlled transactivator rtTA2s-S2. We show that the rtTA2s-S2 is useful for transient inducible expression of genes in embryonic stem cells. However, we found that it was not possible to establish mouse embryonic stem cell lines stably expressing this transactivator. Using the viral IRES sequence which couples the expression of rtTA2s-S2 and neomycin phosphotransferase, we found that embryonic stem cells expressing rtTA2s-S2 are not capable of growing in the presence of G418. Our results indicate that this transactivator is toxic to ES cells and raise the need for the development of other strategies for stable and inducible expression of genes in ES cells. ER -
BRYJA, V., J. PACHERNÍK, L. KUBALA, Aleš HAMPL and P. DVOŘÁK. The reverse tetracycline-controlled transactivator rtTA2s-S2 is toxic in mouse embryonic stem cells. \textit{Reproduction, nutrition, development}. 2003, vol.~43, No~6, p.~477-486. ISSN~0926-5287.
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