The reverse tetracycline-controlled transactivator rtTA2s-S2 is toxic in mouse embryonic stem cells

BRYJA, V., J. PACHERNÍK, L. KUBALA, Aleš HAMPL and P. DVOŘÁK. The reverse tetracycline-controlled transactivator rtTA2s-S2 is toxic in mouse embryonic stem cells. Reproduction, nutrition, development. 2003, vol. 43, No 6, p. 477-486. ISSN 0926-5287.

Basic information

Original name

The reverse tetracycline-controlled transactivator rtTA2s-S2 is toxic in mouse embryonic stem cells

Authors

BRYJA, V., J. PACHERNÍK, L. KUBALA, Aleš HAMPL and P. DVOŘÁK

Edition

Reproduction, nutrition, development, 2003, 0926-5287

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Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

Genetics and molecular biology

Country of publisher

France

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 0.780

Organization unit

Faculty of Science

Keywords in English

stem cells

Tags

stem cells

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Abstract

V originále

The efficient and reversible control of transgene expression is a powerful tool for the correct manipulation of embryonic stem cells in both cell therapy and transgenesis. The aim of this work was to investigate the possibilities of recently developed reverse tetracycline-controlled transactivator rtTA2s-S2. We show that the rtTA2s-S2 is useful for transient inducible expression of genes in embryonic stem cells. However, we found that it was not possible to establish mouse embryonic stem cell lines stably expressing this transactivator. Using the viral IRES sequence which couples the expression of rtTA2s-S2 and neomycin phosphotransferase, we found that embryonic stem cells expressing rtTA2s-S2 are not capable of growing in the presence of G418. Our results indicate that this transactivator is toxic to ES cells and raise the need for the development of other strategies for stable and inducible expression of genes in ES cells.