GFP assay as a sensitive eukaryotic screening model to detect
toxic and genotoxic activity of azaarenes

D 2005

GFP assay as a sensitive eukaryotic screening model to detect toxic and genotoxic activity of azaarenes

BARTOŠ, Tomáš, Stefan LETZSCH, Michal ŠKAREK, Zuzana FLEGROVÁ, Pavel ČUPR et. al.

Basic information

Original name

GFP assay as a sensitive eukaryotic screening model to detect toxic and genotoxic activity of azaarenes

Name in Czech

GFP eukaryotický test

Authors

BARTOŠ, Tomáš (203 Czech Republic, guarantor), Stefan LETZSCH (276 Germany), Michal ŠKAREK (203 Czech Republic), Zuzana FLEGROVÁ (203 Czech Republic), Pavel ČUPR (203 Czech Republic) and Ivan HOLOUBEK (203 Czech Republic)

Edition

Lille, SETAC Europe 15th Annual Meeting, Book of Abstracts, p. 276-276, 2005

Publisher

SETAC

Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

30304 Public and environmental health

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/05:00013139

Organization unit

Faculty of Science

UT WoS

000239364500009

Keywords in English

GFP; genotoxicity; azaarene

Abstract

ORIG CZ

V originále

Azaarenes are nitrogen containing polyaromatic heterocyclic compounds. The majority of the azaarenes found in the environment originate from anthropogenic sources. Concentrations of NPAHs found in the environment are reported to be one to two orders of magnitude lower than PAH concentrations, yet their biological effects can be of similar magnitude. Very few studies on the genotoxicity of azaarenes are available in the literature. In the present study, a preliminary profile of both the toxic and genotoxic potential of 5 PAHs and their 20 aza-analogues were investigated. To assess the toxic and genotoxic activity a GFP assay based on the yeast Saccharomyces cerevisiae was selected. To compare the sensitivity of this eukaryotic short-term assay with bacterial screening tests, the Toxi-Chromotest for toxicity and SOS-Chromotest for genotoxicity assessment were also performed. This comparison indicates that in most cases the yeast GFP assay is apparently of comparable specificity to the bacterial toxicity or genotoxicity tests with respect to the correlation of positive/negative responses, but much more sensitive with respect to the effective concentration values. In the cases of phenazine, phenanthridine, 1,10-phenanthroline or 4,7-phenanthroline one to two orders of magnitude lower EC20 and MGC values in the yeast GFP assay were observed. In this study we present evidence that genotoxicity assessment using the yeast GFP assay can provide a simple system to monitor the activity of these environmental pollutants that could possess mutagenic potential at low concentrations.

In Czech

GFP test

Links

GA525/03/0367, research and development project

Name: Ekotoxikologie persistentních organických polutantů životního prostředí

Investor: Czech Science Foundation, Ecotoxicology of persistent organic environmental pollutants

Citovat

BARTOŠ, Tomáš, Stefan LETZSCH, Michal ŠKAREK, Zuzana FLEGROVÁ, Pavel ČUPR and Ivan HOLOUBEK. GFP assay as a sensitive eukaryotic screening model to detect toxic and genotoxic activity of azaarenes. In SETAC Europe 15th Annual Meeting, Book of Abstracts. Lille: SETAC, 2005, p. 276.

@inproceedings{618506,
   author = {Bartoš, Tomáš and Letzsch, Stefan and Škarek, Michal and Flegrová, Zuzana and Čupr, Pavel and Holoubek, Ivan},
   address = {Lille},
   booktitle = {SETAC Europe 15th Annual Meeting, Book of Abstracts},
   keywords = {GFP; genotoxicity; azaarene},
   language = {eng},
   location = {Lille},
   pages = {276-276},
   publisher = {SETAC},
   title = {GFP assay as a sensitive eukaryotic screening model to detect toxic and genotoxic activity of azaarenes},
   year = {2005}
}

TY  - JOUR
ID  - 618506
AU  - Bartoš, Tomáš - Letzsch, Stefan - Škarek, Michal - Flegrová, Zuzana - Čupr, Pavel - Holoubek, Ivan
PY  - 2005
TI  - GFP assay as a sensitive eukaryotic screening model to detect toxic and genotoxic activity of azaarenes
PB  - SETAC
CY  - Lille
KW  - GFP
KW  - genotoxicity
KW  - azaarene
N2  - Azaarenes are nitrogen containing polyaromatic heterocyclic compounds. The majority of the azaarenes found in the environment originate from anthropogenic sources. Concentrations of NPAHs found in the environment are reported to be one to two orders of magnitude lower than PAH concentrations, yet their biological effects can be of similar magnitude. Very few studies on the genotoxicity of azaarenes are available in the literature. In the present study, a preliminary profile of both the toxic and genotoxic potential of 5 PAHs and their 20 aza-analogues were investigated. To assess the toxic and genotoxic activity a GFP assay based on the yeast Saccharomyces cerevisiae was selected. To compare the sensitivity of this eukaryotic short-term assay with bacterial screening tests, the Toxi-Chromotest for toxicity and SOS-Chromotest for genotoxicity assessment were also performed. This comparison indicates that in most cases the yeast GFP assay is apparently of comparable specificity to the bacterial toxicity or genotoxicity tests with respect to the correlation of positive/negative responses, but much more sensitive with respect to the effective concentration values. In the cases of phenazine, phenanthridine, 1,10-phenanthroline or 4,7-phenanthroline one to two orders of magnitude lower EC20 and MGC values in the yeast GFP assay were observed. In this study we present evidence that genotoxicity assessment using the yeast GFP assay can provide a simple system to monitor the activity of these environmental pollutants that could possess mutagenic potential at low concentrations.
ER  -

BARTOŠ, Tomáš, Stefan LETZSCH, Michal ŠKAREK, Zuzana FLEGROVÁ, Pavel ČUPR and Ivan HOLOUBEK. GFP assay as a sensitive eukaryotic screening model to detect toxic and genotoxic activity of azaarenes. In \textit{SETAC Europe 15th Annual Meeting, Book of Abstracts}. Lille: SETAC, 2005, p.~276.

Detailed Information on Publication Record