| BARTOŠ, Tomáš, Michal ŠKAREK, Pavel ČUPR, Petra KOSUBOVÁ and Ivan HOLOUBEK. Genotoxic activity of a technical toxaphene mixture and its photodegradation products in the SOS genotoxicity tests. MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS. Amsterdam, Netherlands: ELSEVIER SCIENCE BV, 2005, vol. 2005, No 565, p. 113-120. ISSN 1383-5718. |
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@article{618518,
author = {Bartoš, Tomáš and Škarek, Michal and Čupr, Pavel and Kosubová, Petra and Holoubek, Ivan},
article_location = {Amsterdam, Netherlands},
article_number = {565},
keywords = {Toxaphene; genotoxicity assays; SOS response; umuC test; photodegradation; UV-irradiation},
language = {eng},
issn = {1383-5718},
journal = {MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS},
title = {Genotoxic activity of a technical toxaphene mixture and its photodegradation products in the SOS genotoxicity tests},
volume = {2005},
year = {2005}
}
TY - JOUR
ID - 618518
AU - Bartoš, Tomáš - Škarek, Michal - Čupr, Pavel - Kosubová, Petra - Holoubek, Ivan
PY - 2005
TI - Genotoxic activity of a technical toxaphene mixture and its photodegradation products in the SOS genotoxicity tests
JF - MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS
VL - 2005
IS - 565
SP - 113-120
EP - 113-120
PB - ELSEVIER SCIENCE BV
SN - 13835718
KW - Toxaphene
KW - genotoxicity assays
KW - SOS response
KW - umuC test
KW - photodegradation
KW - UV-irradiation
N2 - Toxaphene (CAS No. 800-35-2) is a complex mixture of several hundred components, that was used worldwide primarily as an agricultural pesticide with insecticide effects in the second half of the 20th century. In vitro investigations of genotoxicity and mutagenicity of toxaphene were in literature generally described, but they provided somewhat equivocal results. We re-evaluated the technical toxaphene in two prokaryotic systems. The SOS Chromotest showed high sensitivity to toxaphene. Three highest concentrations (40, 20 and 10 mg/l) were clearly positive and the dose-response effect was evident. In the umuC assay at concentrations from 2.5 to 40.0 mg/l, a dose-dependent increase in genotoxic activity was observed, but these results were found to be insignificant. Genotoxicity of toxaphene and its photodegradation products was also examined in this study after UV irradiation (3-6-9 h) at concentrations from 7.5 to 60.0 mg/l. Irradiated solution of technical toxaphene after 3 h showed no significant evidence of growth inhibition. However exposure of Salmonella to 6-h UV-irradiated toxaphene showed toxic effect compared to negative control. After 9-h irradiation rapid decrease of bacterial growth was observed. Activity of grbeta-galactosidase of toxaphene solution was markedly increased after 6 and 9 h irradiation and significantly reached 2.4-times, respectively 3.1-times higher values relative to control variant and exceeded the criteria of a significant genotoxicity. These results show that while technical toxaphene is a weak, direct-acting mutagen in some bacterial tests, the evidence of dose-dependent toxicity and genotoxicity its photoproducts could be conclusively demonstrated by umuC testing procedure.
ER -
BARTOŠ, Tomáš, Michal ŠKAREK, Pavel ČUPR, Petra KOSUBOVÁ and Ivan HOLOUBEK. Genotoxic activity of a technical toxaphene mixture and its photodegradation products in the SOS genotoxicity tests. \textit{MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS}. Amsterdam, Netherlands: ELSEVIER SCIENCE BV, 2005, vol.~2005, No~565, p.~113-120. ISSN~1383-5718.
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