Detailed Information on Publication Record
2005
Genotoxic activity of a technical toxaphene mixture and its photodegradation products in the SOS genotoxicity tests
BARTOŠ, Tomáš, Michal ŠKAREK, Pavel ČUPR, Petra KOSUBOVÁ, Ivan HOLOUBEK et. al.Basic information
Original name
Genotoxic activity of a technical toxaphene mixture and its photodegradation products in the SOS genotoxicity tests
Name in Czech
Genotoxický potenciál toxafenu a jeho fotodegradačních produktů
Authors
BARTOŠ, Tomáš (203 Czech Republic, guarantor), Michal ŠKAREK (203 Czech Republic), Pavel ČUPR (203 Czech Republic), Petra KOSUBOVÁ (203 Czech Republic) and Ivan HOLOUBEK (203 Czech Republic)
Edition
MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS, Amsterdam, Netherlands, ELSEVIER SCIENCE BV, 2005, 1383-5718
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
30304 Public and environmental health
Country of publisher
Czech Republic
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 2.188
RIV identification code
RIV/00216224:14310/05:00013140
Organization unit
Faculty of Science
UT WoS
000226764600002
Keywords in English
Toxaphene; genotoxicity assays; SOS response; umuC test; photodegradation; UV-irradiation
Změněno: 11/5/2007 09:21, RNDr. Tomáš Bartoš, Ph.D.
V originále
Toxaphene (CAS No. 800-35-2) is a complex mixture of several hundred components, that was used worldwide primarily as an agricultural pesticide with insecticide effects in the second half of the 20th century. In vitro investigations of genotoxicity and mutagenicity of toxaphene were in literature generally described, but they provided somewhat equivocal results. We re-evaluated the technical toxaphene in two prokaryotic systems. The SOS Chromotest showed high sensitivity to toxaphene. Three highest concentrations (40, 20 and 10 mg/l) were clearly positive and the dose-response effect was evident. In the umuC assay at concentrations from 2.5 to 40.0 mg/l, a dose-dependent increase in genotoxic activity was observed, but these results were found to be insignificant. Genotoxicity of toxaphene and its photodegradation products was also examined in this study after UV irradiation (3-6-9 h) at concentrations from 7.5 to 60.0 mg/l. Irradiated solution of technical toxaphene after 3 h showed no significant evidence of growth inhibition. However exposure of Salmonella to 6-h UV-irradiated toxaphene showed toxic effect compared to negative control. After 9-h irradiation rapid decrease of bacterial growth was observed. Activity of grbeta-galactosidase of toxaphene solution was markedly increased after 6 and 9 h irradiation and significantly reached 2.4-times, respectively 3.1-times higher values relative to control variant and exceeded the criteria of a significant genotoxicity. These results show that while technical toxaphene is a weak, direct-acting mutagen in some bacterial tests, the evidence of dose-dependent toxicity and genotoxicity its photoproducts could be conclusively demonstrated by umuC testing procedure.
In Czech
Toxaphene
Links
| GA525/03/0367, research and development project |
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