Detailed Information on Publication Record
2005
The Use of Liquid Chromatography and Capillary Electrophoresis for Determinationof Trinitrotoluenes and Product of their Biotransformation.
BEČANOVÁ, Jitka, Zdeněk ŠIMEK, Věra HERNÍKOVÁ and Zdeněk FRIEDLBasic information
Original name
The Use of Liquid Chromatography and Capillary Electrophoresis for Determinationof Trinitrotoluenes and Product of their Biotransformation.
Name in Czech
Použití kapalinové chromatografie a kapilární elektroforézy pro stanovení trinitrotoluenů a produktů jejich biotransformace
Authors
BEČANOVÁ, Jitka (203 Czech Republic), Zdeněk ŠIMEK (203 Czech Republic, guarantor), Věra HERNÍKOVÁ (203 Czech Republic) and Zdeněk FRIEDL (203 Czech Republic)
Edition
Lille, France, The Raison dEtre of environmental toxicology and chemistry, SETAC Europe 15th Annual Meeting, 22-26 May 2005, p. 203-203, 1 pp. 2005
Publisher
Society of Environmental Toxicology and Chemistry
Other information
Language
English
Type of outcome
Stať ve sborníku
Field of Study
10406 Analytical chemistry
Country of publisher
France
Confidentiality degree
není předmětem státního či obchodního tajemství
RIV identification code
RIV/00216224:14310/05:00014617
Organization unit
Faculty of Science
Keywords in English
Liquid Chromatography; Capillary Electrophoresis; Trinitrotoluenes
Změněno: 31/1/2006 15:21, doc. RNDr. Zdeněk Šimek, CSc.
V originále
Nitroderivatives of toluene such as 2,4,6-trinitrotoluene (TNT) belong to most used explosives. TNT, other trinitrotoluenes and products of their biotranformations, aminodinitrotoluenes (ADNT) and diaminonitrotoluenes (DANT) are widely distributed in the environment. The various nitroaromatic compounds have been found as contaminants in soils at munitions manufacturing facilities, storage depots, and sites of the explosives use. TNT is often stepwise biologically reduced to aminodinitrotoluenes (ADNT) and diaminonitrotoluenes (DANT) in the environment. They have mutagenic, carcinogenic and toxic effects. TNT, 2-ADNT, 4-ADNT, 2,4-DANT, 2,6-DANT have been found to be cytotoxic presumably due to induced oxidative stress. TNT, and the TNT-derived metabolites demonstrate mutagenic capability. According to EPA, TNT is possible human carcinogen. The most commonly used method for the analysis of nitrotoluenes and aminonitrotoluenes is high performance liquid chromatography (HPLC) with UV detection due to its widespread availability. At present, electromigration methods are used due to their unrivalled separation efficiency. Comparison of retention behaviour of selected nitroderivatives of toluene and aminonitroderivatives of toluene in liquid chromatography (HPLC), capillary electrochromatography (CEC) and micellar electrokinetic chromatography (MECC) separation systems is presented. Fused silica capillary filled with octadecylsilica stationary phase (CEC Hypersil C18, 3m, 100 m I.D.) was used for electrochromatography experiments and the standard bare fused silica capillary (50 m I.D.) for micellar electrokinetic chromatography. Effect of pH and concentration of background electrolyte, sodium dodecylsulphate (SDS) concentration and organic modifier content was investigated. Efect of mobile phase composition and different gradient elutions in reversed phase liquid chromatography system was studied. The applicability of separation method under investigation for determination of nitroderivatives of toluene in environmental samples is discused.
In Czech
Nitroderivatives of toluene such as 2,4,6-trinitrotoluene (TNT) belong to most used explosives. TNT, other trinitrotoluenes and products of their biotranformations, aminodinitrotoluenes (ADNT) and diaminonitrotoluenes (DANT) are widely distributed in the environment. The various nitroaromatic compounds have been found as contaminants in soils at munitions manufacturing facilities, storage depots, and sites of the explosives use. TNT is often stepwise biologically reduced to aminodinitrotoluenes (ADNT) and diaminonitrotoluenes (DANT) in the environment. They have mutagenic, carcinogenic and toxic effects. TNT, 2-ADNT, 4-ADNT, 2,4-DANT, 2,6-DANT have been found to be cytotoxic presumably due to induced oxidative stress. TNT, and the TNT-derived metabolites demonstrate mutagenic capability. According to EPA, TNT is possible human carcinogen. The most commonly used method for the analysis of nitrotoluenes and aminonitrotoluenes is high performance liquid chromatography (HPLC) with UV detection due to its widespread availability. At present, electromigration methods are used due to their unrivalled separation efficiency. Comparison of retention behaviour of selected nitroderivatives of toluene and aminonitroderivatives of toluene in liquid chromatography (HPLC), capillary electrochromatography (CEC) and micellar electrokinetic chromatography (MECC) separation systems is presented. Fused silica capillary filled with octadecylsilica stationary phase (CEC Hypersil C18, 3m, 100 m I.D.) was used for electrochromatography experiments and the standard bare fused silica capillary (50 m I.D.) for micellar electrokinetic chromatography. Effect of pH and concentration of background electrolyte, sodium dodecylsulphate (SDS) concentration and organic modifier content was investigated. Efect of mobile phase composition and different gradient elutions in reversed phase liquid chromatography system was studied. The applicability of separation method under investigation for determination of nitroderivatives of toluene in environmental samples is discused.
Links
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