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BEČANOVÁ, Jitka, Zdeněk ŠIMEK, Věra HERNÍKOVÁ and Zdeněk FRIEDL. The Use of Liquid Chromatography and Capillary Electrophoresis for Determinationof Trinitrotoluenes and Product of their Biotransformation. In The Raison dEtre of environmental toxicology and chemistry, SETAC Europe 15th Annual Meeting, 22-26 May 2005. Lille, France: Society of Environmental Toxicology and Chemistry, 2005, p. 203-203.

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TY  - JOUR
ID  - 618589
AU  - Bečanová, Jitka - Šimek, Zdeněk - Herníková, Věra - Friedl, Zdeněk
PY  - 2005
TI  - The Use of Liquid Chromatography and Capillary Electrophoresis for Determinationof Trinitrotoluenes and Product of their Biotransformation.
PB  - Society of Environmental Toxicology and Chemistry
CY  - Lille, France
KW  - Liquid Chromatography
KW  - Capillary Electrophoresis
KW  - Trinitrotoluenes
N2  - Nitroderivatives of toluene such as 2,4,6-trinitrotoluene (TNT) belong to most used explosives. TNT, other trinitrotoluenes and products of their biotranformations, aminodinitrotoluenes (ADNT) and diaminonitrotoluenes (DANT) are widely distributed in the environment. The various nitroaromatic compounds have been found as contaminants in soils at munitions manufacturing facilities, storage depots, and sites of the explosives use. TNT is often stepwise biologically reduced to aminodinitrotoluenes (ADNT) and diaminonitrotoluenes (DANT) in the environment. They have mutagenic, carcinogenic and toxic effects. TNT, 2-ADNT, 4-ADNT, 2,4-DANT, 2,6-DANT have been found to be cytotoxic presumably due to induced oxidative stress. TNT, and the TNT-derived metabolites demonstrate mutagenic capability. According to EPA, TNT is possible human carcinogen. The most commonly used method for the analysis of nitrotoluenes and aminonitrotoluenes is high performance liquid chromatography (HPLC) with UV detection due to its widespread availability. At present, electromigration methods are used due to their unrivalled separation efficiency. Comparison of retention behaviour of selected nitroderivatives of toluene and aminonitroderivatives of toluene in liquid chromatography (HPLC), capillary electrochromatography (CEC) and micellar electrokinetic chromatography (MECC) separation systems is presented. Fused silica capillary filled with octadecylsilica stationary phase (CEC Hypersil C18, 3m, 100 m I.D.) was used for electrochromatography experiments and the standard bare fused silica capillary (50 m I.D.) for micellar electrokinetic chromatography. Effect of pH and concentration of background electrolyte, sodium dodecylsulphate (SDS) concentration and organic modifier content was investigated. Efect of mobile phase composition and different gradient elutions in reversed phase liquid chromatography system was studied. The applicability of separation method under investigation for determination of nitroderivatives of toluene in environmental samples is discused.
ER  -

Basic information
Original name	The Use of Liquid Chromatography and Capillary Electrophoresis for Determinationof Trinitrotoluenes and Product of their Biotransformation.
Name in Czech	Použití kapalinové chromatografie a kapilární elektroforézy pro stanovení trinitrotoluenů a produktů jejich biotransformace
Authors	BEČANOVÁ, Jitka (203 Czech Republic), Zdeněk ŠIMEK (203 Czech Republic, guarantor), Věra HERNÍKOVÁ (203 Czech Republic) and Zdeněk FRIEDL (203 Czech Republic).
Edition	Lille, France, The Raison dEtre of environmental toxicology and chemistry, SETAC Europe 15th Annual Meeting, 22-26 May 2005, p. 203-203, 1 pp. 2005.
Publisher	Society of Environmental Toxicology and Chemistry

Other information
Original language	English
Type of outcome	Proceedings paper
Field of Study	10406 Analytical chemistry
Country of publisher	France
Confidentiality degree	is not subject to a state or trade secret
RIV identification code	RIV/00216224:14310/05:00014617
Organization unit	Faculty of Science
Keywords in English	Liquid Chromatography; Capillary Electrophoresis; Trinitrotoluenes
Tags	Capillary electrophoresis, liquid chromatography, Trinitrotoluenes
Changed by	Changed by: doc. RNDr. Zdeněk Šimek, CSc., učo 37019. Changed: 31/1/2006 15:21.

Abstract
Nitroderivatives of toluene such as 2,4,6-trinitrotoluene (TNT) belong to most used explosives. TNT, other trinitrotoluenes and products of their biotranformations, aminodinitrotoluenes (ADNT) and diaminonitrotoluenes (DANT) are widely distributed in the environment. The various nitroaromatic compounds have been found as contaminants in soils at munitions manufacturing facilities, storage depots, and sites of the explosives use. TNT is often stepwise biologically reduced to aminodinitrotoluenes (ADNT) and diaminonitrotoluenes (DANT) in the environment. They have mutagenic, carcinogenic and toxic effects. TNT, 2-ADNT, 4-ADNT, 2,4-DANT, 2,6-DANT have been found to be cytotoxic presumably due to induced oxidative stress. TNT, and the TNT-derived metabolites demonstrate mutagenic capability. According to EPA, TNT is possible human carcinogen. The most commonly used method for the analysis of nitrotoluenes and aminonitrotoluenes is high performance liquid chromatography (HPLC) with UV detection due to its widespread availability. At present, electromigration methods are used due to their unrivalled separation efficiency. Comparison of retention behaviour of selected nitroderivatives of toluene and aminonitroderivatives of toluene in liquid chromatography (HPLC), capillary electrochromatography (CEC) and micellar electrokinetic chromatography (MECC) separation systems is presented. Fused silica capillary filled with octadecylsilica stationary phase (CEC Hypersil C18, 3m, 100 m I.D.) was used for electrochromatography experiments and the standard bare fused silica capillary (50 m I.D.) for micellar electrokinetic chromatography. Effect of pH and concentration of background electrolyte, sodium dodecylsulphate (SDS) concentration and organic modifier content was investigated. Efect of mobile phase composition and different gradient elutions in reversed phase liquid chromatography system was studied. The applicability of separation method under investigation for determination of nitroderivatives of toluene in environmental samples is discused.

Abstract

Nitroderivatives of toluene such as 2,4,6-trinitrotoluene (TNT) belong to most used explosives. TNT, other trinitrotoluenes and products of their biotranformations, aminodinitrotoluenes (ADNT) and diaminonitrotoluenes (DANT) are widely distributed in the environment. The various nitroaromatic compounds have been found as contaminants in soils at munitions manufacturing facilities, storage depots, and sites of the explosives use. TNT is often stepwise biologically reduced to aminodinitrotoluenes (ADNT) and diaminonitrotoluenes (DANT) in the environment. They have mutagenic, carcinogenic and toxic effects. TNT, 2-ADNT, 4-ADNT, 2,4-DANT, 2,6-DANT have been found to be cytotoxic presumably due to induced oxidative stress. TNT, and the TNT-derived metabolites demonstrate mutagenic capability. According to EPA, TNT is possible human carcinogen. The most commonly used method for the analysis of nitrotoluenes and aminonitrotoluenes is high performance liquid chromatography (HPLC) with UV detection due to its widespread availability. At present, electromigration methods are used due to their unrivalled separation efficiency. Comparison of retention behaviour of selected nitroderivatives of toluene and aminonitroderivatives of toluene in liquid chromatography (HPLC), capillary electrochromatography (CEC) and micellar electrokinetic chromatography (MECC) separation systems is presented. Fused silica capillary filled with octadecylsilica stationary phase (CEC Hypersil C18, 3m, 100 m I.D.) was used for electrochromatography experiments and the standard bare fused silica capillary (50 m I.D.) for micellar electrokinetic chromatography. Effect of pH and concentration of background electrolyte, sodium dodecylsulphate (SDS) concentration and organic modifier content was investigated. Efect of mobile phase composition and different gradient elutions in reversed phase liquid chromatography system was studied. The applicability of separation method under investigation for determination of nitroderivatives of toluene in environmental samples is discused.

Abstract (in Czech)
Nitroderivatives of toluene such as 2,4,6-trinitrotoluene (TNT) belong to most used explosives. TNT, other trinitrotoluenes and products of their biotranformations, aminodinitrotoluenes (ADNT) and diaminonitrotoluenes (DANT) are widely distributed in the environment. The various nitroaromatic compounds have been found as contaminants in soils at munitions manufacturing facilities, storage depots, and sites of the explosives use. TNT is often stepwise biologically reduced to aminodinitrotoluenes (ADNT) and diaminonitrotoluenes (DANT) in the environment. They have mutagenic, carcinogenic and toxic effects. TNT, 2-ADNT, 4-ADNT, 2,4-DANT, 2,6-DANT have been found to be cytotoxic presumably due to induced oxidative stress. TNT, and the TNT-derived metabolites demonstrate mutagenic capability. According to EPA, TNT is possible human carcinogen. The most commonly used method for the analysis of nitrotoluenes and aminonitrotoluenes is high performance liquid chromatography (HPLC) with UV detection due to its widespread availability. At present, electromigration methods are used due to their unrivalled separation efficiency. Comparison of retention behaviour of selected nitroderivatives of toluene and aminonitroderivatives of toluene in liquid chromatography (HPLC), capillary electrochromatography (CEC) and micellar electrokinetic chromatography (MECC) separation systems is presented. Fused silica capillary filled with octadecylsilica stationary phase (CEC Hypersil C18, 3m, 100 m I.D.) was used for electrochromatography experiments and the standard bare fused silica capillary (50 m I.D.) for micellar electrokinetic chromatography. Effect of pH and concentration of background electrolyte, sodium dodecylsulphate (SDS) concentration and organic modifier content was investigated. Efect of mobile phase composition and different gradient elutions in reversed phase liquid chromatography system was studied. The applicability of separation method under investigation for determination of nitroderivatives of toluene in environmental samples is discused.

Abstract (in Czech)

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MSM0021622412, plan (intention)	Name: Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální, regionální a lokální úrovni (INCHEMBIOL) (Acronym: INCHEMBIOL)
MSM0021622412, plan (intention)	Investor: Ministry of Education, Youth and Sports of the CR, Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL)