The yield of chromosomal aberrations in children with acute
leukemia using methods GTG banding, FISH, CGH and HR-CGH

D 2005

The yield of chromosomal aberrations in children with acute leukemia using methods GTG banding, FISH, CGH and HR-CGH

MENTZLOVÁ, Dita, Alexandra OLTOVÁ, Vlasta LINKOVÁ, Petr KUGLÍK, Jaroslav ŠTĚRBA et. al.

Basic information

Original name

The yield of chromosomal aberrations in children with acute leukemia using methods GTG banding, FISH, CGH and HR-CGH

Name in Czech

Chromozomové aberace u dětí s akutní leukémií studované pomocí technik G-pruhování, fluorescenční hybridizace in situ, komparativní genomové hybridizace a komparativní genomové hybridizace s vysokým rozlišením

Authors

MENTZLOVÁ, Dita (203 Czech Republic), Alexandra OLTOVÁ (203 Czech Republic), Vlasta LINKOVÁ (203 Czech Republic), Petr KUGLÍK (203 Czech Republic, guarantor), Jaroslav ŠTĚRBA (203 Czech Republic), Martina PEŠÁKOVÁ (203 Czech Republic), Vladimíra VRANOVÁ (703 Slovakia), Denisa MENDELOVÁ (203 Czech Republic), Dita ŽEŽULKOVÁ (203 Czech Republic) and Hana FILKOVÁ (203 Czech Republic)

Edition

Praha, European Journal of Human Genetics, p. 187-187, 2005

Publisher

Nature Publishing Group

Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

Genetics and molecular biology

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 3.251

RIV identification code

RIV/00216224:14310/05:00014618

Organization unit

Faculty of Science

ISSN

Keywords in English

Acute leukemia; chromosome aberrations; cytogenetics; FISH; CGH; HR-CGH

Abstract

ORIG CZ

V originále

We report results from the study of 33 children with acute lymphoid (25) and myeloid (8) leukaemia. Karyotyping of bone marrow cells is an important step for the precise diagnosis and for the choice of the adequate treatment in leukaemic patients. Twenty six patients were examined in the time of diagnosis and seven in relapse. The cytogenetic analyses were performed on 24-hours bone marrow cells cultured in RPMI 1640 according to the standard methods. GTG banding technique revealed clonal karyotypic abnormalities in 45% (15) samples. Cytogenetic findings were confirmed by fluorescence in situ hybridization (FISH) method. Comparative genomic hybridization (CGH) was used on bone marrow samples obtained from this patients in time of diagnosis or relapse. CGH showed new DNA copy number changes in 70% (23) patients. For the first time we were tested the modification of CGH. We show examples of submicroscopic chromosomal imbalances being detected with this high resolution CGH technique (HR-CGH). By HR-CGH we detected new chromosomal aberation in more than 75% cases. Here we present comparing among GTG-band karyotyping, CGH and HR-CGH results.

In Czech

Práce srovnává výtěžnost detekce specifických chromozomových abnormalit u 33 dětských pacientů s akutní leukémií pomocí techniky G-pruhování, FISH, CGH a HR-CGH.

Links

MSM0021622415, plan (intention)

Name: Molekulární podstata buněčných a tkáňových regulací

Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations

Citovat

MENTZLOVÁ, Dita, Alexandra OLTOVÁ, Vlasta LINKOVÁ, Petr KUGLÍK, Jaroslav ŠTĚRBA, Martina PEŠÁKOVÁ, Vladimíra VRANOVÁ, Denisa MENDELOVÁ, Dita ŽEŽULKOVÁ and Hana FILKOVÁ. The yield of chromosomal aberrations in children with acute leukemia using methods GTG banding, FISH, CGH and HR-CGH. In European Journal of Human Genetics. Praha: Nature Publishing Group, 2005, p. 187. ISSN 1476-5438.

@inproceedings{618592,
   author = {Mentzlová, Dita and Oltová, Alexandra and Linková, Vlasta and Kuglík, Petr and Štěrba, Jaroslav and Pešáková, Martina and Vranová, Vladimíra and Mendelová, Denisa and Žežulková, Dita and Filková, Hana},
   address = {Praha},
   booktitle = {European Journal of Human Genetics},
   keywords = {Acute leukemia; chromosome aberrations; cytogenetics; FISH; CGH; HR-CGH},
   language = {eng},
   location = {Praha},
   pages = {187-187},
   publisher = {Nature Publishing Group},
   title = {The yield of chromosomal aberrations in children with acute leukemia using methods GTG banding, FISH, CGH and HR-CGH},
   year = {2005}
}

TY  - JOUR
ID  - 618592
AU  - Mentzlová, Dita - Oltová, Alexandra - Linková, Vlasta - Kuglík, Petr - Štěrba, Jaroslav - Pešáková, Martina - Vranová, Vladimíra - Mendelová, Denisa - Žežulková, Dita - Filková, Hana
PY  - 2005
TI  - The yield of chromosomal aberrations in children with acute leukemia using methods GTG banding, FISH, CGH and HR-CGH
PB  - Nature Publishing Group
CY  - Praha
KW  - Acute leukemia
KW  - chromosome aberrations
KW  - cytogenetics
KW  - FISH
KW  - CGH
KW  - HR-CGH
N2  - We report results from the study of 33 children with acute lymphoid (25) and myeloid (8) leukaemia. Karyotyping of bone marrow cells is an important step for the precise diagnosis and for the choice of the adequate treatment in leukaemic patients. Twenty six patients were examined in the time of diagnosis and seven in relapse. The cytogenetic analyses were performed on 24-hours bone marrow cells cultured in RPMI 1640 according to the standard methods. GTG banding technique revealed clonal karyotypic abnormalities in 45% (15) samples. Cytogenetic findings were confirmed by fluorescence in situ hybridization (FISH) method. Comparative genomic hybridization (CGH) was used on bone marrow samples obtained from this patients in time of diagnosis or relapse. CGH showed new DNA copy number changes in 70% (23) patients. For the first time we were tested the modification of CGH. We show examples of submicroscopic chromosomal imbalances being detected with this high resolution CGH technique (HR-CGH). By HR-CGH we detected new chromosomal aberation in more than 75% cases. Here we present comparing among GTG-band karyotyping, CGH and HR-CGH results.
ER  -

MENTZLOVÁ, Dita, Alexandra OLTOVÁ, Vlasta LINKOVÁ, Petr KUGLÍK, Jaroslav ŠTĚRBA, Martina PEŠÁKOVÁ, Vladimíra VRANOVÁ, Denisa MENDELOVÁ, Dita ŽEŽULKOVÁ and Hana FILKOVÁ. The yield of chromosomal aberrations in children with acute leukemia using methods GTG banding, FISH, CGH and HR-CGH. In \textit{European Journal of Human Genetics}. Praha: Nature Publishing Group, 2005, p.~187. ISSN~1476-5438.

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