Elimination  voltammetry  with	linear	scan  as  a  new
detection  method   for   DNA	sensors

J 2005

Elimination voltammetry with linear scan as a new detection method for DNA sensors

TRNKOVÁ, Libuše, František JELEN, Jitka PETRLOVÁ, Vojtěch ADAM, David POTĚŠIL et. al.

Basic information

Original name

Elimination voltammetry with linear scan as a new detection method for DNA sensors

Name in Czech

Eliminační voltametrie s lineárním skenem jako nová detekční metoda pro DNA senzory

Authors

TRNKOVÁ, Libuše (203 Czech Republic, guarantor), František JELEN (203 Czech Republic), Jitka PETRLOVÁ (203 Czech Republic), Vojtěch ADAM (203 Czech Republic), David POTĚŠIL (203 Czech Republic) and René KIZEK (203 Czech Republic)

Edition

SENSORS, 2005, 1424-8220

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10405 Electrochemistry

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 1.208

RIV identification code

RIV/00216224:14310/05:00014629

Organization unit

Faculty of Science

UT WoS

000233333900009

Keywords in English

oligodeoxynucleotide; DNA biosensor; linear sweep voltammetry; elimination voltammetry with linear scan; reduction of adenine and cytosine; mercury electrode; sequence of nucleotides

Abstract

ORIG CZ

V originále

The paper describes successful coupling of adsorptive transfer stripping (AdTS) and elimination voltammetry with linear scan (EVLS) for the resolution of reduction signals of cytosine ( C) and adenine ( A) residues in hetero-oligodeoxynucleotides (ODNs). Short ODNs (9-mers and 20-mers) were adsorbed from a small volume on a hanging mercury drop electrode (HMDE). After washing of the ODN-modified electrode by water and its transferring to an electrochemical cell, voltammetric curves were measured. The AdTS EVLS was able to determine of C/A ratio of ODNs through the elimination function conserving the diffusion current component and eliminating kinetic and charging current components. This function, which provides the elimination signal in a peak-counterpeak form, increased the current sensitivity for A and C resolution, and for the recognition of bases sequences in ODN chains. Optimal conditions of elimination experiments such as pH, time of adsorption, and scan rate were found. The combination of EVLS with AdTS procedure can be considered as a new detection method in a DNA sensor.

In Czech

Tato práce popistuje

Links

MSM0021622412, plan (intention)

Name: Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální, regionální a lokální úrovni (INCHEMBIOL) (Acronym: INCHEMBIOL)

Investor: Ministry of Education, Youth and Sports of the CR, Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL)

Citovat

TRNKOVÁ, Libuše, František JELEN, Jitka PETRLOVÁ, Vojtěch ADAM, David POTĚŠIL and René KIZEK. Elimination voltammetry with linear scan as a new detection method for DNA sensors. SENSORS. 2005, vol. 5, 6-10, p. 448-464. ISSN 1424-8220.

@article{618643,
   author = {Trnková, Libuše and Jelen, František and Petrlová, Jitka and Adam, Vojtěch and Potěšil, David and Kizek, René},
   article_number = {6-10},
   keywords = {oligodeoxynucleotide; DNA biosensor; linear sweep voltammetry; elimination voltammetry with linear scan; reduction of adenine and cytosine; mercury electrode; sequence of nucleotides},
   language = {eng},
   issn = {1424-8220},
   journal = {SENSORS},
   title = {Elimination voltammetry with linear scan as a new detection method for DNA sensors},
   volume = {5},
   year = {2005}
}

TY  - JOUR
ID  - 618643
AU  - Trnková, Libuše - Jelen, František - Petrlová, Jitka - Adam, Vojtěch - Potěšil, David - Kizek, René
PY  - 2005
TI  - Elimination voltammetry with linear scan as a new detection method for DNA sensors
JF  - SENSORS
VL  - 5
IS  - 6-10
SP  - 448-464
EP  - 448-464
SN  - 14248220
KW  - oligodeoxynucleotide
KW  - DNA biosensor
KW  - linear sweep voltammetry
KW  - elimination voltammetry with linear scan
KW  - reduction of adenine and cytosine
KW  - mercury electrode
KW  - sequence of nucleotides
N2  - The paper describes successful coupling of adsorptive transfer stripping (AdTS) and elimination voltammetry with linear scan (EVLS) for the resolution of reduction signals of cytosine ( C) and adenine ( A) residues in hetero-oligodeoxynucleotides (ODNs). Short ODNs (9-mers and 20-mers) were adsorbed from a small volume on a hanging mercury drop electrode (HMDE). After washing of the ODN-modified electrode by water and its transferring to an electrochemical cell, voltammetric curves were measured. The AdTS EVLS was able to determine of C/A ratio of ODNs through the elimination function conserving the diffusion current component and eliminating kinetic and charging current components. This function, which provides the elimination signal in a peak-counterpeak form, increased the current sensitivity for A and C resolution, and for the recognition of bases sequences in ODN chains. Optimal conditions of elimination experiments such as pH, time of adsorption, and scan rate were found. The combination of EVLS with AdTS procedure can be considered as a new detection method in a DNA sensor.
ER  -

TRNKOVÁ, Libuše, František JELEN, Jitka PETRLOVÁ, Vojtěch ADAM, David POTĚŠIL and René KIZEK. Elimination voltammetry with linear scan as a new detection method for DNA sensors. \textit{SENSORS}. 2005, vol.~5, 6-10, p.~448-464. ISSN~1424-8220.

Detailed Information on Publication Record