Determination of Trinitrotoluenes and product of their Biotransformation using liquid chromatography and Capillary Electrophoresis

BEČANOVÁ, Jitka, Zdeněk ŠIMEK and Zdeněk FRIEDL. Determination of Trinitrotoluenes and product of their Biotransformation using liquid chromatography and Capillary Electrophoresis. Chemické Listy. Praha: Česká společnost chemická, 2005, roč. 99, 99(s), p. 121-123. ISSN 0009-2770.

Basic information

• Original name: Determination of Trinitrotoluenes and product of their Biotransformation using liquid chromatography and Capillary Electrophoresis
• Name in Czech: Stanovení trinitrotoluenů a produktů jejich biotransformace kapalinovou chromatografií a kapilární elektroforézou
• Authors: BEČANOVÁ, Jitka (203 Czech Republic), Zdeněk ŠIMEK (203 Czech Republic, guarantor) and Zdeněk FRIEDL (203 Czech Republic).
• Edition: Chemické Listy, Praha, Česká společnost chemická, 2005, 0009-2770.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 10406 Analytical chemistry
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• Impact factor: Impact factor: 0.445
• RIV identification code: RIV/00216224:14310/05:00014637
• Organization unit: Faculty of Science
• Keywords in English: Chromatogrphy; Electrophoresis; Trinitrotoluene
• Tags: Chromatogrphy, Electrophoresis, Trinitrotoluene

Abstract

Contamination by the explosive as 2,4,6-trinitrotoluene (TNT), is often associated with former and present munitions manufacturing facilities, storage depots, and former sites of explosives use.1 TNT is widely distributed in enviroment and there can be persistent for very long times or slowly degradation in the water and in the soil, influenced by light, microorganisms, oxygen and reducing chemicals. TNT and their products of degradation, aminodinitrotoluenes (ADNT), diaminonitrotoluenes (DANT) and triaminotoluene (TAT) have been found to be cytotoxic presumably due to induced oxidative stress and demonstrate mutagenic capability (EPA classifies TNT as possible human carcinogens).2,3 Complete separation of these structurally similar compounds is necessary in order to their accurately determine and quantify in environmental samples and in (bio)transformation studies. High performance liquid chromatography (HPLC) has remained the major analytical tool for the detection and quantification of nitroaromatic compounds.4 At present, capillary electromigration methods, capillary electrochromatography (CEC) and micellar electrokinetic chromatography (MEKC) enable to separate these compound with good separation efficiency.

Abstract (in Czech)

Contamination by the explosive as 2,4,6-trinitrotoluene (TNT), is often associated with former and present munitions manufacturing facilities, storage depots, and former sites of explosives use.1 TNT is widely distributed in enviroment and there can be persistent for very long times or slowly degradation in the water and in the soil, influenced by light, microorganisms, oxygen and reducing chemicals. TNT and their products of degradation, aminodinitrotoluenes (ADNT), diaminonitrotoluenes (DANT) and triaminotoluene (TAT) have been found to be cytotoxic presumably due to induced oxidative stress and demonstrate mutagenic capability (EPA classifies TNT as possible human carcinogens).2,3 Complete separation of these structurally similar compounds is necessary in order to their accurately determine and quantify in environmental samples and in (bio)transformation studies. High performance liquid chromatography (HPLC) has remained the major analytical tool for the detection and quantification of nitroaromatic compounds.4 At present, capillary electromigration methods, capillary electrochromatography (CEC) and micellar electrokinetic chromatography (MEKC) enable to separate these compound with good separation efficiency.

Links

• MSM0021622412, plan (intention): Name: Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální, regionální a lokální úrovni (INCHEMBIOL) (Acronym: INCHEMBIOL)

Investor: Ministry of Education, Youth and Sports of the CR, Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL)