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@inproceedings{619105, author = {Hofman, Jakub and Švihálek, Jiří and Nečas, Martin}, address = {České Budějovice, Czech Republic}, booktitle = {Proceedings of the 9th Methodological workshop: Present methods for investigation of microbial community biodiversity in soils and substrates}, keywords = {soil; microorganisms; diversity; BIOLOG}, language = {eng}, location = {České Budějovice, Czech Republic}, isbn = {80-86525-03-1}, pages = {67-72}, publisher = {Institute of Soil Biology AS CR}, title = {Evaluating the functional diversity of soil microorganisms using the BIOLOG method in situ studies and laboratory tests of toxicity}, year = {2004} }
TY - JOUR ID - 619105 AU - Hofman, Jakub - Švihálek, Jiří - Nečas, Martin PY - 2004 TI - Evaluating the functional diversity of soil microorganisms using the BIOLOG method in situ studies and laboratory tests of toxicity PB - Institute of Soil Biology AS CR CY - České Budějovice, Czech Republic SN - 8086525031 KW - soil KW - microorganisms KW - diversity KW - BIOLOG N2 - This contribution is short example of one field study and three laboratory experiments, which were carried out to answer the question, if the BIOLOG method is suitable to be sensitive indicator of soil contamination in soil ecotoxicology. The BIOLOG main difficulty is introduced especially as effect of inoculum density which causes that information about overall catabolic activity cannot be separated from information about functional diversity, i.e. differences in substrate utilization patterns. In our case field study, twelve soils were measured from area around an industrial source. Soils were compared separately according their catabolic activity and relative diversity. In laboratory toxicity tests, cadmium for validation and toxaphene or chlorinated paraffin as problematic environmental pollutants were evaluated. Cadmium validated BIOLOG to be sensitive as overall physiological activity endpoint. However, there were no diversity changes induced. Toxaphene revealed no effect even at 10 ppm level. The highest levels (10 000 ppm) of both chlorinated paraffin and dodecane stimulated physiological activity and also induced some changes in physiological profile. To conclude, BIOLOG is promising for soil ecotoxicology studies but separated assessment of differences in physiological activity from differences in functional diversity, i.e. substrate utilization patterns, are strongly required to interpret the data correctly. ER -
HOFMAN, Jakub, Jiří ŠVIHÁLEK and Martin NEČAS. Evaluating the functional diversity of soil microorganisms using the BIOLOG method in situ studies and laboratory tests of toxicity. In \textit{Proceedings of the 9th Methodological workshop: Present methods for investigation of microbial community biodiversity in soils and substrates}. České Budějovice, Czech Republic: Institute of Soil Biology AS CR, 2004, p.~67-72. ISBN~80-86525-03-1.
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