Toxicity and biochemical responses og higher plants after exposure to N-heterocyclic PAHs and their unsubstituted analogues

PAŠKOVÁ, Veronika, Klára HILSCHEROVÁ and Luděk BLÁHA. Toxicity and biochemical responses og higher plants after exposure to N-heterocyclic PAHs and their unsubstituted analogues. In Book of abstracts of ECOTOX 2005 - Advances and Trends in Ecotoxicology. 1st ed. Brno: Masaryk University Brno, 2005, p. 187-187, 248 pp. ISBN 80-210-3799-7.

Basic information

• Original name: Toxicity and biochemical responses og higher plants after exposure to N-heterocyclic PAHs and their unsubstituted analogues
• Name in Czech: Toxicita a modulace biochemických markerů u vyšších rostlin po expozici N-PAHs a jejich nesubstituovaných analogů
• Authors: PAŠKOVÁ, Veronika (203 Czech Republic, guarantor), Klára HILSCHEROVÁ (203 Czech Republic) and Luděk BLÁHA (203 Czech Republic).
• Edition: 1st ed. Brno, Book of abstracts of ECOTOX 2005 - Advances and Trends in Ecotoxicology, p. 187-187, 248 pp. 2005.
• Publisher: Masaryk University Brno

Other information

• Original language: English
• Type of outcome: Proceedings paper
• Field of Study: 10600 1.6 Biological sciences
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• RIV identification code: RIV/00216224:14310/05:00014707
• Organization unit: Faculty of Science
• ISBN: 80-210-3799-7
• Keywords in English: phytotoxicity; Sinapis alba; Triticum aestivum; Phaseolus vulgaris; PAHs; N-PAHs; lipid peroxidation; glutathione; glutathione-S-transferase; glutathione reductase; glutathione peroxidase
• Tags: Glutathione, glutathione peroxidase, glutathione reductase, glutathione-S-transferase, lipid peroxidation, N-PAHs, PaHs, Phaseolus vulgaris, phytotoxicity, Sinapis alba, Triticum aestivum

Abstract

In framework of this diploma thesis were studied toxic effects of polyaromatic hydrocarbons (phenanthrene, anthracene, fluorene) and its N-heterocyclic derivates (phenanthridine, 1,10-phenanthroline, 4,7-phenanthroline, 1,7-phenanthroline, benzo[h]quinoline, acridine, carbazole) on terrestrial plants white mustard (Sinapis alba), wheat (Triticum aestivum) and bean (Phaseolus vulgaris). Tests of acute phytotoxicity were carried out in laboratories of the centre RECETOX, Masaryk University in Brno. The classic morphological parametres (shoot length, hypocotyl length and their weights) and germinability of seeds were observed. The parameter of lipid peroxidation (TBARS), as well as the level and activity of antioxidative components of plant metabolism (glutathione, glutathione-S-transferase, glutathione reductase, glutathione peroxidase) were measured to identify the rate of induction of oxidative stress. Evidently new informations about in the meanwhile little studied group of environmental toxicants (PAHs versus aza-PAHs) were established in this work. The results of this study implicate that homocyclic polyaromatic hydrocarbons are less toxic than their N-substituents, with respect to interference in morphological parametres. S. alba and T. aestivum were more sensitive than P. vulgaris. Chemicals with the strongest inhibition effect on the process of germination and the growth of plants were determined (phenanthridine, acridine, benzo[h]quinoline, 1,10 - and 1,7-phenanthroline). Furthermore were found out that fluorene, carbazole, acridine, phenanthrene, phenanthridine, benzo[h]quinoline and 1,7-phenanthroline significantly increased lipid peroxidation. All tested chemicals significantly induced the increase of activity of detoxification plant enzymes, in case of increased level of glutathion significantly caused all tested chemicals except 1,10- and 4,7-phenanthroline.

Abstract (in Czech)

V rámci práce byly studovány toxické efekty polyaromatických uhlovodík (fenantren, antracen, fluoren) a jejich N-heterocyklických derivát (fenantridin, 1,10-fenantrolin, 1,7-fenantrolin, 4,7-fenantrolin, benzo[h]chinolin, akridin, karbazol) na suchozemských rostlinách hoici bílé (Sinapis alba), pšenici seté (Triticum aestivum) a fazolu obecném (Phaseolus vulgaris). V laboratoi centra RECETOX, Masarykova univerzita Brno, byl proveden akutní test fytotoxicity a sledovány klasické morfologické parametry (délka koene a hypokotylu a jejich hmotnost) a klíivost. Pro posouzení míry indukce oxidativního stresu byl men parametr lipidní peroxidace (TBARS) a sledovány hladiny a aktivity antioxidativních složek rostlinného metabolismu (glutathion, glutathion-S-transferáza, glutathion reduktáza a glutathion peroxidáza). V této studii byly zjištny zejm nové poznatky o zatím málo prostudované skupin environmentálních toxikant (PAHs versus aza-PAHs). Z výsledk vyplývá, že homocyklické PAHs jsou mén toxické než jejich N-substituenty, pokud jde o ovlivnní morfologických parametr. S.alba a T.aestivum jsou citlivjší než P.vulgaris. Byly ureny látky s nejvtším inhibiním efektem na proces klíení semen a rst rostlin (fenantridin, akridin, benzo[h]chinolin, 1,10- a 1,7-fenantrolin). Dále bylo zjištno, že lipidní peroxidaci významn indukují fluoren, karbazol, akridin, fenantren, fenantridin, benzo[h]chinolin a 1,7-fenantrolin. Všechny testované látky významn indukovaly nárst aktivit detoxifikaních enzym rostlin, v pípad glutathionu mli významný efekt na zvýšení hladiny všechny testované látky krom 1,10- a 4,7-fenantrolinu.

Links

• MSM0021622412, plan (intention): Name: Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální, regionální a lokální úrovni (INCHEMBIOL) (Acronym: INCHEMBIOL)

Investor: Ministry of Education, Youth and Sports of the CR, Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL)