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ANGER, M., V. BRYJA, L. JIRMANOVÁ, Aleš HAMPL, J. MOTLÍK, P. DVOŘÁK and M. KUBELKA. The appearance of truncated cyclin A2 correlates with differentiation of mouse embryonic stem cells. Biochemical and biophysical research communications, 2003, vol. 302, No 4, p. 825-830. ISSN 0006-291X.
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Basic information
Original name The appearance of truncated cyclin A2 correlates with differentiation of mouse embryonic stem cells
Authors ANGER, M., V. BRYJA, L. JIRMANOVÁ, Aleš HAMPL, J. MOTLÍK, P. DVOŘÁK and M. KUBELKA.
Edition Biochemical and biophysical research communications, 2003, 0006-291X.
Other information
Original language English
Type of outcome Article in a journal
Field of Study Genetics and molecular biology
Country of publisher United States of America
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 2.836
Organization unit Faculty of Science
UT WoS 000181881300029
Keywords in English stem cells
Tags stem cells
Changed by Changed by: prof. Mgr. Vítězslav Bryja, Ph.D., učo 11088. Changed: 8/7/2009 10:59.
Abstract
The presence of a form of cyclin A2 with an N-terminal truncation has recently been reported in various murine cell lines and tissues. The truncated cyclin A2 binds to and activates the cyclin-dependent kinase 2 (CDK2). However, CDK2 bound by the truncated cyclin A2 is located in the cytoplasm in contrast to CDK2 bound to full-length cyclin A2, which is in the nucleus. Here, we show that proliferating mouse embryonic stem cells (ES cells) contain very little truncated cyclin A2 but as the cells are induced to differentiate the amount of truncated cyclin A2 increases. The expression pattern of truncated cyclin A2 was the same in p27(Kip1) -/- differentiating ES cells as in the differentiating wild-type cells. We conclude that p27(Kip1) is not necessary for the proteolytic cleavage that gives rise to the truncated form of cyclin A2 in differentiating ES cells and that this post-translational modification is not a function of the cell density but is correlated with differentiation.
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