J 2003

The appearance of truncated cyclin A2 correlates with differentiation of mouse embryonic stem cells

ANGER, M., V. BRYJA, L. JIRMANOVÁ, Aleš HAMPL, J. MOTLÍK et. al.

Basic information

Original name

The appearance of truncated cyclin A2 correlates with differentiation of mouse embryonic stem cells

Authors

ANGER, M., V. BRYJA, L. JIRMANOVÁ, Aleš HAMPL, J. MOTLÍK, P. DVOŘÁK and M. KUBELKA

Edition

Biochemical and biophysical research communications, 2003, 0006-291X

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

Genetics and molecular biology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 2.836

Organization unit

Faculty of Science

UT WoS

000181881300029

Keywords in English

stem cells

Tags

Změněno: 8/7/2009 10:59, prof. Mgr. Vítězslav Bryja, Ph.D.

Abstract

V originále

The presence of a form of cyclin A2 with an N-terminal truncation has recently been reported in various murine cell lines and tissues. The truncated cyclin A2 binds to and activates the cyclin-dependent kinase 2 (CDK2). However, CDK2 bound by the truncated cyclin A2 is located in the cytoplasm in contrast to CDK2 bound to full-length cyclin A2, which is in the nucleus. Here, we show that proliferating mouse embryonic stem cells (ES cells) contain very little truncated cyclin A2 but as the cells are induced to differentiate the amount of truncated cyclin A2 increases. The expression pattern of truncated cyclin A2 was the same in p27(Kip1) -/- differentiating ES cells as in the differentiating wild-type cells. We conclude that p27(Kip1) is not necessary for the proteolytic cleavage that gives rise to the truncated form of cyclin A2 in differentiating ES cells and that this post-translational modification is not a function of the cell density but is correlated with differentiation.