ANGER, M., V. BRYJA, L. JIRMANOVÁ, Aleš HAMPL, J. MOTLÍK, P. DVOŘÁK a M. KUBELKA. The appearance of truncated cyclin A2 correlates with differentiation of mouse embryonic stem cells. Biochemical and biophysical research communications, 2003, roč. 302, č. 4, s. 825-830. ISSN 0006-291X.
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Základní údaje
Originální název The appearance of truncated cyclin A2 correlates with differentiation of mouse embryonic stem cells
Autoři ANGER, M., V. BRYJA, L. JIRMANOVÁ, Aleš HAMPL, J. MOTLÍK, P. DVOŘÁK a M. KUBELKA.
Vydání Biochemical and biophysical research communications, 2003, 0006-291X.
Další údaje
Originální jazyk angličtina
Typ výsledku Článek v odborném periodiku
Obor Genetika a molekulární biologie
Stát vydavatele Spojené státy
Utajení není předmětem státního či obchodního tajemství
Impakt faktor Impact factor: 2.836
Organizační jednotka Přírodovědecká fakulta
UT WoS 000181881300029
Klíčová slova anglicky stem cells
Štítky stem cells
Změnil Změnil: prof. Mgr. Vítězslav Bryja, Ph.D., učo 11088. Změněno: 8. 7. 2009 10:59.
Anotace
The presence of a form of cyclin A2 with an N-terminal truncation has recently been reported in various murine cell lines and tissues. The truncated cyclin A2 binds to and activates the cyclin-dependent kinase 2 (CDK2). However, CDK2 bound by the truncated cyclin A2 is located in the cytoplasm in contrast to CDK2 bound to full-length cyclin A2, which is in the nucleus. Here, we show that proliferating mouse embryonic stem cells (ES cells) contain very little truncated cyclin A2 but as the cells are induced to differentiate the amount of truncated cyclin A2 increases. The expression pattern of truncated cyclin A2 was the same in p27(Kip1) -/- differentiating ES cells as in the differentiating wild-type cells. We conclude that p27(Kip1) is not necessary for the proteolytic cleavage that gives rise to the truncated form of cyclin A2 in differentiating ES cells and that this post-translational modification is not a function of the cell density but is correlated with differentiation.
VytisknoutZobrazeno: 13. 8. 2020 02:57