Komparativní genomika Treponema pallidum technikou genomové hybridizace na oligonukleotidovém čipu.

MATĚJKOVÁ, Petra and David ŠMAJS. Komparativní genomika Treponema pallidum technikou genomové hybridizace na oligonukleotidovém čipu. (Comparative genomics of T. pallidum strains Nichols and SS14 on the oligonucleotide array). In Abstrakta 49. Studentské vědecké konference. 2005.

Basic information

• Original name: Komparativní genomika Treponema pallidum technikou genomové hybridizace na oligonukleotidovém čipu.
• Name (in English): Comparative genomics of T. pallidum strains Nichols and SS14 on the oligonucleotide array
• Authors: MATĚJKOVÁ, Petra (203 Czech Republic) and David ŠMAJS (203 Czech Republic, guarantor).
• Edition: Abstrakta 49. Studentské vědecké konference, 2005.

Other information

• Original language: Czech
• Type of outcome: Conference abstract
• Field of Study: Genetics and molecular biology
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• RIV identification code: RIV/00216224:14110/05:00013195
• Organization unit: Faculty of Medicine
• Keywords in English: Treponema pallidum ssp. pallidum; Nichols; SS14
• Tags: Nichols, SS14, Treponema pallidum ssp. pallidum

Abstract

Cíl: Cílem práce bylo sekvenovat genom Treponema pallidum subsp. pallidum SS14 a porovnat získanou kompletní genomovou sekvenci se sekvencí T. pallidum subsp. pallidum Nichols. Oba porovnávané kmeny způsobují pohlavně přenosné onemocnění syfilis. Celkem bylo v genomu kmene SS14 nalezeno 329 SNP, 7 delecí a 4 inzerce.

Abstract (in English)

The genome of Treponema pallidum ssp. pallidum strain SS14, the causative agent of syphilis, was sequenced using oligonucleotide arrays. The sequences of more than 49 kb of the SS14 genome were verified by capillary sequencing approach. When compared to T. pallidum ssp. pallidum strain Nichols, the genome of SS14 contained 329 single nucleotide changes (219 transitions and 104 transversions), 7 deletions and 4 insertions.

Links

• GA310/04/0021, research and development project: Name: Postgenomické studie syfilitické spirochety Treponema pallidum

Investor: Czech Science Foundation, Postgenomic studies on Treponema pallidum, the syphilis spirochete

• MSM0021622415, plan (intention): Name: Molekulární podstata buněčných a tkáňových regulací

Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations

• NI7351, research and development project: Name: Komparativní genomika patogenních spirochet rodu Treponema: cesta k sekvenčně specifické diagnostice

Investor: Ministry of Health of the CR