Comparing genomes of pathogenic and non-pathogenic spirochetes: DNA-microarray hybridizations, whole genome fingerprinting and DNA sequencing.

ŠMAJS, David, Petra MATĚJKOVÁ, Michal STROUHAL, S.J. NORRIS and G.M. WEINSTOCK. Comparing genomes of pathogenic and non-pathogenic spirochetes: DNA-microarray hybridizations, whole genome fingerprinting and DNA sequencing. In Abstract Book of NATO Advanced Research Workshop on Molecular Biology of Spirochetes. 2005.

Basic information

Original name

Comparing genomes of pathogenic and non-pathogenic spirochetes: DNA-microarray hybridizations, whole genome fingerprinting and DNA sequencing.

Name in Czech

Srovnání genomů patogenních a nepatogenních spirochet pomocí DNA-microarray hybridizace, whole genome fingerprintingu a DNA sekvenování.

Authors

ŠMAJS, David (203 Czech Republic, guarantor), Petra MATĚJKOVÁ (203 Czech Republic), Michal STROUHAL (203 Czech Republic), S.J. NORRIS (840 United States of America) and G.M. WEINSTOCK (840 United States of America)

Edition

Abstract Book of NATO Advanced Research Workshop on Molecular Biology of Spirochetes, 2005

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Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

Genetics and molecular biology

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14110/05:00013211

Organization unit

Faculty of Medicine

Keywords in English

Treponema pallidum ssp. pallidum; Treponema pallidum ssp. pertenue; Treponema paraluiscuniculi

Tags

Treponema pallidum ssp. pallidum, Treponema pallidum ssp. pertenue, Treponema paraluiscuniculi

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Abstract

V originále

Genomes of closely related treponemes, Treponema pallidum ssp. pallidum (strains Nichols and SS14) and Treponema pallidum ssp. pertenue (Samoan D) were compared with the genome of Treponema paraluiscuniculi (strain Cuniculi A) using comparative genomic techniques. T. pallidum ssp. pallidum is the causative agent of highly invasive human disease syphilis, T. pallidum ssp. pertenue is the causative agent of yaws and T. paraluiscuniculi is not pathogenic to humans. When compared to Nichols genome, 1, 8 and 34 differences in the BamH I target restriction sites and 3, 7 and 20 indels were found in the genome of SS14, Samoan D and T. paraluiscuniculi, respectively.

In Czech

Genomy blízce příbuzných treponem, Treponema pallidum ssp. pallidum (kmenů Nichols a SS14) a Treponema pallidum ssp. pertenue (Samoan D) byly porovnány s genomem Treponema paraluiscuniculi (kmenCuniculi A) s použitím technik komparativní genomiky. Ve srovnání s kmenem Nichols jsme nalezli, 1, 8 a 34 změn pro cílovou sekvenci restriktázy BamH I a 3, 7 a 20 inzercí a delecí v genomech SS14, Samoan D a T. paraluiscuniculi.

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Links

GA310/04/0021, research and development project	Name: Postgenomické studie syfilitické spirochety Treponema pallidum Investor: Czech Science Foundation, Postgenomic studies on Treponema pallidum, the syphilis spirochete
MSM0021622415, plan (intention)	Name: Molekulární podstata buněčných a tkáňových regulací Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations
NI7351, research and development project	Name: Komparativní genomika patogenních spirochet rodu Treponema: cesta k sekvenčně specifické diagnostice Investor: Ministry of Health of the CR