Reproducibility of Comparative Genomics Sequencing in Treponema pallidum ssp. pallidum.
Name in Czech
Spolehlivost komparativního genomového sekvenování u Treponema pallidum ssp. pallidum.
Authors
MATĚJKOVÁ, Petra (203 Czech Republic), David ŠMAJS (203 Czech Republic, guarantor), T.J. ALBERT (840 United States of America), S.J. NORRIS (840 United States of America) and G.M. WEINSTOCK (840 United States of America)
Edition
Abstract Book of NATO Advanced Research Workshop on Molecular Biology of Spirochetes, 2005
Other information
Language
English
Type of outcome
Konferenční abstrakt
Field of Study
Genetics and molecular biology
Country of publisher
Czech Republic
Confidentiality degree
není předmětem státního či obchodního tajemství
RIV identification code
RIV/00216224:14110/05:00013212
Organization unit
Faculty of Medicine
Keywords in English
T. pallidum subspecies pallidum; strain SS14; Comparative Genomic Sequencing
The genome of T. pallidum subspecies pallidum strain SS14 was sequenced twice using Comparative Genomic Sequencing (CGS) strategy. In the first mapping stage, fluorescently labeled genomic Two independent CGS experiments with different DNA isolates of SS14 strain (4950 and 4951) were performed. 319 and 317 SNPs were identified with 4951 and 4950 DNA isolations, respectively. Both sequencing experiments differed in 14 SNPs, (1 difference per 81 kb of the genome sequence).
In Czech
Genom T. pallidum subspecies pallidum kmene SS14 byl dvakrát nezávisle sekvenován s použitím strategie CGS - Comparative Genomic Sequencing strategy. Dva nezávislé experimenty s odlišnými vzorky DNA kmene SS14 (4950 a 4951) odhalily 319 a 317 SNP zámn. Oba experimenty se lišily ve 14 SNP, (1 rozdíl na 81 kb genomové sekvence).
Links
GA310/04/0021, research and development project
Name: Postgenomické studie syfilitické spirochety Treponema pallidum
Investor: Czech Science Foundation, Postgenomic studies on Treponema pallidum, the syphilis spirochete
MSM0021622415, plan (intention)
Name: Molekulární podstata buněčných a tkáňových regulací
Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations
NI7351, research and development project
Name: Komparativní genomika patogenních spirochet rodu Treponema: cesta k sekvenčně specifické diagnostice