Reproducibility of Comparative Genomics Sequencing in Treponema pallidum ssp. pallidum.
MATĚJKOVÁ, Petra, David ŠMAJS, T.J. ALBERT, S.J. NORRIS and G.M. WEINSTOCK. Reproducibility of Comparative Genomics Sequencing in Treponema pallidum ssp. pallidum. In Abstract Book of NATO Advanced Research Workshop on Molecular Biology of Spirochetes. 2005. |
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Basic information | |
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Original name | Reproducibility of Comparative Genomics Sequencing in Treponema pallidum ssp. pallidum. |
Name in Czech | Spolehlivost komparativního genomového sekvenování u Treponema pallidum ssp. pallidum. |
Authors | MATĚJKOVÁ, Petra (203 Czech Republic), David ŠMAJS (203 Czech Republic, guarantor), T.J. ALBERT (840 United States of America), S.J. NORRIS (840 United States of America) and G.M. WEINSTOCK (840 United States of America). |
Edition | Abstract Book of NATO Advanced Research Workshop on Molecular Biology of Spirochetes, 2005. |
Other information | |
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Original language | English |
Type of outcome | Conference abstract |
Field of Study | Genetics and molecular biology |
Country of publisher | Czech Republic |
Confidentiality degree | is not subject to a state or trade secret |
RIV identification code | RIV/00216224:14110/05:00013212 |
Organization unit | Faculty of Medicine |
Keywords in English | T. pallidum subspecies pallidum; strain SS14; Comparative Genomic Sequencing |
Tags | Comparative Genomic Sequencing, strain SS14, T. pallidum subspecies pallidum |
Changed by | Changed by: prof. MUDr. David Šmajs, Ph.D., učo 1116. Changed: 8/4/2010 17:17. |
Abstract |
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The genome of T. pallidum subspecies pallidum strain SS14 was sequenced twice using Comparative Genomic Sequencing (CGS) strategy. In the first mapping stage, fluorescently labeled genomic Two independent CGS experiments with different DNA isolates of SS14 strain (4950 and 4951) were performed. 319 and 317 SNPs were identified with 4951 and 4950 DNA isolations, respectively. Both sequencing experiments differed in 14 SNPs, (1 difference per 81 kb of the genome sequence). |
Abstract (in Czech) |
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Genom T. pallidum subspecies pallidum kmene SS14 byl dvakrát nezávisle sekvenován s použitím strategie CGS - Comparative Genomic Sequencing strategy. Dva nezávislé experimenty s odlišnými vzorky DNA kmene SS14 (4950 a 4951) odhalily 319 a 317 SNP zámn. Oba experimenty se lišily ve 14 SNP, (1 rozdíl na 81 kb genomové sekvence). |
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GA310/04/0021, research and development project | Name: Postgenomické studie syfilitické spirochety Treponema pallidum |
Investor: Czech Science Foundation, Postgenomic studies on Treponema pallidum, the syphilis spirochete | |
MSM0021622415, plan (intention) | Name: Molekulární podstata buněčných a tkáňových regulací |
Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations | |
NI7351, research and development project | Name: Komparativní genomika patogenních spirochet rodu Treponema: cesta k sekvenčně specifické diagnostice |
Investor: Ministry of Health of the CR |
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