JANSOVÁ, Eva, Irena KOUTNÁ, Petr KRONTORÁD, Zbyněk SVOBODA, Silvie KŘIVÁNKOVÁ, Jan ŽALOUDÍK, Michal KOZUBEK and Stanislav KOZUBEK. Comparative transcriptome maps: a new approach to the diagnosis of colorectal carcinoma patients using cDNA microarrays. Clinical Genetics. Denmark: Blackwell Munksgaard, 2006, vol. 69, No 3, p. 218-227. ISSN 0009-9163. |
Other formats:
BibTeX
LaTeX
RIS
@article{622604, author = {Jansová, Eva and Koutná, Irena and Krontorád, Petr and Svoboda, Zbyněk and Křivánková, Silvie and Žaloudík, Jan and Kozubek, Michal and Kozubek, Stanislav}, article_location = {Denmark}, article_number = {3}, keywords = {colorectal carcinoma; cDNA microarrays; transcriptome map; functional clustering; expression profiles}, language = {eng}, issn = {0009-9163}, journal = {Clinical Genetics}, title = {Comparative transcriptome maps: a new approach to the diagnosis of colorectal carcinoma patients using cDNA microarrays}, volume = {69}, year = {2006} }
TY - JOUR ID - 622604 AU - Jansová, Eva - Koutná, Irena - Krontorád, Petr - Svoboda, Zbyněk - Křivánková, Silvie - Žaloudík, Jan - Kozubek, Michal - Kozubek, Stanislav PY - 2006 TI - Comparative transcriptome maps: a new approach to the diagnosis of colorectal carcinoma patients using cDNA microarrays JF - Clinical Genetics VL - 69 IS - 3 SP - 218-227 EP - 218-227 PB - Blackwell Munksgaard SN - 00099163 KW - colorectal carcinoma KW - cDNA microarrays KW - transcriptome map KW - functional clustering KW - expression profiles N2 - The progression of colorectal cancer involves accumulation of various genetic and epigenetic events that dramatically change gene expression. The aim of this study was to investigate a possible new approach to the diagnosis of colorectal carcinoma patients, based on their gene expression profiles. Human 19K cDNA microarrays were used to analyze the gene expression profiles of 18 colorectal carcinoma patients. Transcriptome maps (TM) were analyzed to detect chromosomal regions that could serve as potential diagnostic markers for colon cancer. A comparison of TMs showed chromosome regions with conserved changes of gene expression typical of colorectal cancer in general, and also patient-specific variable regions. We identified 195 genes with significantly altered expression in colon cancer. Functional analysis of the regulated genes distinguished three main categories: biological processes, cellular components, and molecular functions. We found that different patients had chromosome regions characterized by very similar changes of gene expression, probably linked to the most fundamental events in carcinogenesis. On the other hand, variable chromosome regions can be patient-specific. The variable regions may provide further information on the individual pathogenesis and prognosis of the patient. Comparison of TMs is proposed as a tool to facilitate diagnosis and treatment planning for individual patients. ER -
JANSOVÁ, Eva, Irena KOUTNÁ, Petr KRONTORÁD, Zbyněk SVOBODA, Silvie KŘIVÁNKOVÁ, Jan ŽALOUDÍK, Michal KOZUBEK and Stanislav KOZUBEK. Comparative transcriptome maps: a new approach to the diagnosis of colorectal carcinoma patients using cDNA microarrays. \textit{Clinical Genetics}. Denmark: Blackwell Munksgaard, 2006, vol.~69, No~3, p.~218-227. ISSN~0009-9163.
|