Detailed Information on Publication Record
2006
Comparative transcriptome maps: a new approach to the diagnosis of colorectal carcinoma patients using cDNA microarrays
JANSOVÁ, Eva, Irena KOUTNÁ, Petr KRONTORÁD, Zbyněk SVOBODA, Silvie KŘIVÁNKOVÁ et. al.Basic information
Original name
Comparative transcriptome maps: a new approach to the diagnosis of colorectal carcinoma patients using cDNA microarrays
Name in Czech
Srovnávací transkripční mapy: nový přístup v diagnostice pacientů s kolorektálním karcinomem pomocí cDNA microarrays
Authors
JANSOVÁ, Eva (203 Czech Republic, guarantor), Irena KOUTNÁ (203 Czech Republic), Petr KRONTORÁD (203 Czech Republic), Zbyněk SVOBODA (203 Czech Republic), Silvie KŘIVÁNKOVÁ (203 Czech Republic), Jan ŽALOUDÍK (203 Czech Republic), Michal KOZUBEK (203 Czech Republic) and Stanislav KOZUBEK (203 Czech Republic)
Edition
Clinical Genetics, Denmark, Blackwell Munksgaard, 2006, 0009-9163
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
Genetics and molecular biology
Country of publisher
Denmark
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 3.140
RIV identification code
RIV/00216224:14330/06:00015299
Organization unit
Faculty of Informatics
UT WoS
000235840300006
Keywords in English
colorectal carcinoma; cDNA microarrays; transcriptome map; functional clustering; expression profiles
Tags
Tags
International impact, Reviewed
Změněno: 7/5/2010 09:05, prof. RNDr. Michal Kozubek, Ph.D.
V originále
The progression of colorectal cancer involves accumulation of various genetic and epigenetic events that dramatically change gene expression. The aim of this study was to investigate a possible new approach to the diagnosis of colorectal carcinoma patients, based on their gene expression profiles. Human 19K cDNA microarrays were used to analyze the gene expression profiles of 18 colorectal carcinoma patients. Transcriptome maps (TM) were analyzed to detect chromosomal regions that could serve as potential diagnostic markers for colon cancer. A comparison of TMs showed chromosome regions with conserved changes of gene expression typical of colorectal cancer in general, and also patient-specific variable regions. We identified 195 genes with significantly altered expression in colon cancer. Functional analysis of the regulated genes distinguished three main categories: biological processes, cellular components, and molecular functions. We found that different patients had chromosome regions characterized by very similar changes of gene expression, probably linked to the most fundamental events in carcinogenesis. On the other hand, variable chromosome regions can be patient-specific. The variable regions may provide further information on the individual pathogenesis and prognosis of the patient. Comparison of TMs is proposed as a tool to facilitate diagnosis and treatment planning for individual patients.
In Czech
Tento článek se zabývá využitím srovnávacích transkripčních mapjako nového přístupu v diagnostice pacientů s kolorektálním karcinomem s využitím cDNA microarrays.
Links
| GA202/04/0907, research and development project |
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| IAA5004306, research and development project |
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| MSM0021622419, plan (intention) |
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| 1A8241, research and development project |
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