Relationship between genetic polymorphisms in the GPx1, MnSOD
and CAT genes and clinical manifestation of increased oxidative
stress following tracheotomy - preliminary results

D 2005

Relationship between genetic polymorphisms in the GPx1, MnSOD and CAT genes and clinical manifestation of increased oxidative stress following tracheotomy - preliminary results

SALZMAN, Richard, Jan ROTTENBERG, Rom KOSTŘICA, Lukáš PÁCAL, Kateřina KAŇKOVÁ et. al.

Basic information

Original name

Relationship between genetic polymorphisms in the GPx1, MnSOD and CAT genes and clinical manifestation of increased oxidative stress following tracheotomy - preliminary results

Name in Czech

Vztah mezi variantami polymorfizmů genů pro GPx1, MnSOD CAT a klinická manifestace zvýšeného oxidativního stresu po tracheotomii - předběžné výsledky

Authors

SALZMAN, Richard, Jan ROTTENBERG, Rom KOSTŘICA, Lukáš PÁCAL, Kateřina KAŇKOVÁ, Josef TOMANDL and Zuzana HORÁKOVÁ

Edition

1. vyd. Brno, Sborník abstrakt 68. kongresu České společnosti pro otorinolaryngologii a chirurgii hlavy a krku, 52. kongresu Slovenskej spoločnosti pre otorinolaryngológiu a chirurgiu hlavy a krku, p. 109-109, 2005

Publisher

Medica Publishing and Consulting s.r.o.

Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

30200 3.2 Clinical medicine

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

Organization unit

Faculty of Medicine

ISBN

80-239-511

Keywords in English

oxidativní stres; antioxidativní enzymy

Abstract

ORIG CZ

V originále

Objective: Enhanced oxidative stress following tracheobronchial surgery is presumed to have a profound impact on the patients postoperative course. A large number of factors are involved in the process of maintaining the pro-/antioxidative balance in humans with genetic variability in antioxidant enzymes being one of them. The aim of the study was to investigate relationship between selected substitutions in genes encoding for antioxidant enzymes (GPx, MnSOD, CAT) and severity of acute tracheobronchitis following tracheotomy. Materials & Methods: Prospective study of 44 patients after tracheotomy. Indications for tracheotomy were: scheduled surgery for the diagnosis of HNSCC, urgent tracheotomy due to the respiratory insufficiency following the obstruction of upper airways by HNSCC (n=40) or obstruction of an upper respiratory tract caused by other reasons than tumour. Parameters describing the postoperative course plus results of an endoscopic examination of inflammatory changes in tracheobronchial tree were used for calculation of the tracheobronchitis score identified as KTBscore. Endoscopic evaluation of tracheobronchitis severity was scored as the Endoscore. Genotypes of the GPx1 Pro197Leu, MnSOD Ala16Val and CAT -262C/T polymorphisms were detected by means of PCR-based methodology. Results: Allele frequencies were 62.5% Pro, 37.5% Leu of the GPx1 Pro197Leu; 42.9% Ala, 57.1% Val of the MnSOD Ala16Val and 74.3% C allele, 25.7% T allele of the CAT -262C/T. Genotype frequencies of all three polymorphisms studied fulfilled criteria of Hardy-Weinberg equilibrium (P=0.05, chi2 test). Comparison of mean KTBscore, Endoscore and type of cultivated bacteria among particular genotype groups revealed no significant differences (P=0.05, Kruskal-Wallis ANOVA). Conclusions: Based on these pilot results, there is no indication that polymorphism in selected genes encoding for antioxidant enzymes influence both the postoperative course and development of tracheobronchitis following tracheotomy. However, number of subjects enrolled in the study so far represents only a fraction of the planned total number of probands (approx. 240). Therefore, any conclusions drawn so far have to be considered preliminary and speculative. Acknowledgement: Supported by the grant 10/II NR/7996-3 from The Internal Grant Agency of The Ministry of Health of Czech Republic.

In Czech

Cílem práce bylo stanovit vztah mezi vybranými variantami genetických polymorfizmů pro antioxidativní enzymy (GPx, MnSOD, CAT)a závažností průběhu akutní tracheobronchitidy po tracheotomii. Do studie jsme zařadili 44 pacientů po tarcheotomii z následujících indikací: v rámci plánovaného onkochirurgického výkonu pro spinocelulární karcinomy hlavy a krku, akutní zajištění dýchacích cest u pacientů s karcinomy hlavy a krku a obstrukce dýchacích cest u pacientů bez nádorů. na základě těchto pilotních výsledků zatím nemáme indicie pro výrazný vliv genetických polymorfizmů pro antioxidativní enzymy na pooperační průběh po tracheotomii. Vzhledem k nízkému počtu pacientů ve studii je nutné i tyto výslekdy pokládat za předběžné. Prace byla podpořena grantem IGA NR/7996-3.

Links

NR7996, research and development project

Name: Polymorfismus antioxidativních enzymů ve vztahu ke krustózní tracheobronchitidě jako závažné komplikaci u tracheotomovaných pacientů

Investor: Ministry of Health of the CR

Citovat

SALZMAN, Richard, Jan ROTTENBERG, Rom KOSTŘICA, Lukáš PÁCAL, Kateřina KAŇKOVÁ, Josef TOMANDL and Zuzana HORÁKOVÁ. Relationship between genetic polymorphisms in the GPx1, MnSOD and CAT genes and clinical manifestation of increased oxidative stress following tracheotomy - preliminary results. In Sborník abstrakt 68. kongresu České společnosti pro otorinolaryngologii a chirurgii hlavy a krku, 52. kongresu Slovenskej spoločnosti pre otorinolaryngológiu a chirurgiu hlavy a krku. 1st ed. Brno: Medica Publishing and Consulting s.r.o., 2005, p. 109. ISBN 80-239-511.

@inproceedings{623405,
   author = {Salzman, Richard and Rottenberg, Jan and Kostřica, Rom and Pácal, Lukáš and Kaňková, Kateřina and Tomandl, Josef and Horáková, Zuzana},
   address = {Brno},
   booktitle = {Sborník abstrakt 68. kongresu České společnosti pro otorinolaryngologii a chirurgii hlavy a krku, 52. kongresu Slovenskej spoločnosti pre otorinolaryngológiu a chirurgiu hlavy a krku},
   edition = {1.},
   keywords = {oxidativní stres; antioxidativní enzymy},
   language = {eng},
   location = {Brno},
   isbn = {80-239-511},
   pages = {109-109},
   publisher = {Medica Publishing and Consulting s.r.o.},
   title = {Relationship between genetic polymorphisms in the GPx1, MnSOD and CAT genes and clinical manifestation of increased oxidative stress following tracheotomy - preliminary results},
   year = {2005}
}

TY  - JOUR
ID  - 623405
AU  - Salzman, Richard - Rottenberg, Jan - Kostřica, Rom - Pácal, Lukáš - Kaňková, Kateřina - Tomandl, Josef - Horáková, Zuzana
PY  - 2005
TI  - Relationship between genetic polymorphisms in the GPx1, MnSOD and CAT genes and clinical manifestation of increased oxidative stress following tracheotomy - preliminary results
PB  - Medica Publishing and Consulting s.r.o.
CY  - Brno
SN  - 80239511
KW  - oxidativní stres
KW  - antioxidativní enzymy
N2  - Objective: Enhanced oxidative stress following tracheobronchial surgery is presumed to have a profound impact on the patients postoperative course. A large number of factors are involved in the process of maintaining the pro-/antioxidative balance in humans with genetic variability in antioxidant enzymes being one of them. The aim of the study was to investigate relationship between selected substitutions in genes encoding for antioxidant enzymes (GPx, MnSOD, CAT) and severity of acute tracheobronchitis following tracheotomy. Materials & Methods: Prospective study of 44 patients after tracheotomy. Indications for tracheotomy were: scheduled surgery for the diagnosis of HNSCC, urgent tracheotomy due to the respiratory insufficiency following the obstruction of upper airways by HNSCC (n=40) or obstruction of an upper respiratory tract caused by other reasons than tumour. Parameters describing the postoperative course plus results of an endoscopic examination of inflammatory changes in tracheobronchial tree were used for calculation of the tracheobronchitis score identified as KTBscore. Endoscopic evaluation of tracheobronchitis severity was scored as the Endoscore. Genotypes of the GPx1 Pro197Leu, MnSOD Ala16Val and CAT -262C/T polymorphisms were detected by means of PCR-based methodology. Results: Allele frequencies were 62.5% Pro, 37.5% Leu of the GPx1 Pro197Leu; 42.9% Ala, 57.1% Val of the MnSOD Ala16Val and 74.3% C allele, 25.7% T allele of the CAT -262C/T. Genotype frequencies of all three polymorphisms studied fulfilled criteria of Hardy-Weinberg equilibrium (P=0.05, chi2 test). Comparison of mean KTBscore, Endoscore and type of cultivated bacteria among particular genotype groups revealed no significant differences (P=0.05, Kruskal-Wallis ANOVA). Conclusions: Based on these pilot results, there is no indication that polymorphism in selected genes encoding for antioxidant enzymes influence both the postoperative course and development of tracheobronchitis following tracheotomy. However, number of subjects enrolled in the study so far represents only a fraction of the planned total number of probands (approx. 240). Therefore, any conclusions drawn so far have to be considered preliminary and speculative. Acknowledgement: Supported by the grant 10/II NR/7996-3 from The Internal Grant Agency of The Ministry of Health of Czech Republic.
ER  -

SALZMAN, Richard, Jan ROTTENBERG, Rom KOSTŘICA, Lukáš PÁCAL, Kateřina KAŇKOVÁ, Josef TOMANDL and Zuzana HORÁKOVÁ. Relationship between genetic polymorphisms in the GPx1, MnSOD and CAT genes and clinical manifestation of increased oxidative stress following tracheotomy - preliminary results. In \textit{Sborník abstrakt 68. kongresu České společnosti pro otorinolaryngologii a chirurgii hlavy a krku, 52. kongresu Slovenskej spoločnosti pre otorinolaryngológiu a chirurgiu hlavy a krku}. 1st ed. Brno: Medica Publishing and Consulting s.r.o., 2005, p.~109. ISBN~80-239-511.

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