2005
The identification of Gyrodactylus species parasitising Barbatula barbatula L., 1758 in Central Europe: combination morphologic and molecular methods.
PŘIKRYLOVÁ, Iva, Iveta MATĚJUSOVÁ, Jií JARKOVSKÝ and Milan GELNARBasic information
Original name
The identification of Gyrodactylus species parasitising Barbatula barbatula L., 1758 in Central Europe: combination morphologic and molecular methods.
Name in Czech
Identifikace druh cizopasník rodu Gyrodactylus parazitujících na Barbatula barbatula ve stední Evrop kombinací morfologických a molekulárních metod.
Authors
PŘIKRYLOVÁ, Iva (203 Czech Republic), Iveta MATĚJUSOVÁ (203 Czech Republic), Jií JARKOVSKÝ (203 Czech Republic) and Milan GELNAR (203 Czech Republic, guarantor)
Edition
Guangzhou, 5th International Symposium on Monogenea, p. 99-99, 1 pp. 2005
Publisher
Sun Yat-Sen University
Other information
Language
English
Type of outcome
Proceedings paper
Field of Study
10600 1.6 Biological sciences
Country of publisher
China
Confidentiality degree
is not subject to a state or trade secret
RIV identification code
RIV/00216224:14330/05:00013259
Organization unit
Faculty of Informatics
Keywords in English
Gyrodactylus; Barbatula barbatula
Changed: 27/4/2006 20:32, RNDr. JUDr. Vladimír Šmíd, CSc.
V originále
During 2001, Gyrodactylus specimens were collected from Barbatula barbatula originated in Haná River and two sampling sites on Vlára River, Czech Republic, in monthly samples of fifteen fishes. Thirty-one specimens of Gyrodactylus were from different seasons were stored for molecular analysis. Sequencing of the complete internal transcribed spacer (ITS) rRNA genes of Gyrodactylus specimens parasitising B. barbatula from locality Haná revealed sequence differences on the species level. According to the sequence comparison, the presence of at least three Gyrodactylus jiroveci-like species was predicted. Further analysis was required to confirm existence these species according to standard morpholometrical description, using drawings and measurements of the hard part of parasite haptor. According to the shape of marginal hook sickle, the specimens were divided into 3 groups. Measurements of the hard parts of haptor (ten characters) from two species-groups (80 specimens from four seasons) were analysed. Using K mean cluster test and PCA plot, two species-groups were discriminated into two isolated group. Results of this preliminary analysis suggest presence of G. jiroveci and Gyrodactylus papernai (recently used as synonymum for G. jiroveci) in central europeans rivers. The third species-group was determined as Gyrodactylus pseudonemachili that is originally described from Asia, and this species seemed to occurred very rarely in Central Europe. Due to limited number of collected specimens of G. pseudonemachili, this species was not included in metrical analyses.
In Czech
Kombinace molekulárn genetických metod a morfometrických analých je nezbytná pi studii a determinaci živorodých monogeneí rodu Gyrodactylus.
Links
| GD524/05/H536, research and development project |
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| MSM0021622416, plan (intention) |
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