Detailed Information on Publication Record
2005
Biological effects of benzo[c]phenanthridine alkaloids.
SLUNSKÁ, Zdenka, Iva SLANINOVÁ, Jiri SINKORA, Marcela VLKOVA, Eva TÁBORSKÁ et. al.Basic information
Original name
Biological effects of benzo[c]phenanthridine alkaloids.
Name in Czech
Biologické aktivity benzo[c]phenanthridinových alkaloidů
Authors
SLUNSKÁ, Zdenka (203 Czech Republic), Iva SLANINOVÁ (203 Czech Republic, guarantor), Jiri SINKORA (203 Czech Republic), Marcela VLKOVA (203 Czech Republic) and Eva TÁBORSKÁ (203 Czech Republic)
Edition
Volume 3. České Budějovice, CR, Journal of Applied Biomedicine, p. 42-43, 2 pp. 2005
Publisher
University of South Bohemia, Czech Repub
Other information
Language
English
Type of outcome
Stať ve sborníku
Field of Study
Genetics and molecular biology
Country of publisher
Czech Republic
Confidentiality degree
není předmětem státního či obchodního tajemství
RIV identification code
RIV/00216224:14110/05:00013264
Organization unit
Faculty of Medicine
ISBN
1214-021X
Keywords in English
benzo[c]phenanthridine alkaloids; apoptosis; cytotoxicity; MTT assay
Změněno: 19/1/2007 09:53, prof. MUDr. Iva Slaninová, Ph.D.
V originále
Quaternary benzo[c]phenanthridine alkaloids (QBA) are group of natural products, which are discussed as potencial cytostatics for cancer treatment. QBA are relatively small group of isoquinoline alkaloids that have phenylalanin as their precursor. They are distributed in a number of plant species of Papaveraceae, Fumariaceae and Rutaceae families. Sanguinarin and chelerythrin are the two best known QBA and their biological activities have been widely studied (1,2,3). In addition to them about 10 other QBA differing only by substitution on the aromatic rings have been isolated as a minor components. The informations about biological effects of this minor QBA are rare. In this study the differential cytotoxicity of sanguinarine (SA), chelerythrine (CHE) and their minority derivates sanguirubine (SR), chelirubine (CHR) and macarpine (MA) was assessed in vitro by MTT assay. One normal (skin fibroblasts) and 3 tumour cell lines (HeLa; A 431; HL-60) were used as a model object. After 72 hours, MTT assay was performed to evaluate the in vitro cytotoxicity induced by tested alkaloids. Our results show that HL-60 and skin fibroblasts were the most sensitive cell culture lines, while the carcinoma cell lines HeLa and A-431 appeared much more resistant. For apoptosis detection morphology of nuclei of alkaloid treated HL-60 cells after DAPI staining was studied and flow cytometric analysis using Annexin V-FITC and PI was applied. All tested alkaloids appeared to be inductors of apoptosis. Based on our observations we concluded that alkaloids tested in this study show strong antiproliferative and proapoptotic effect in vitro. This work was supported by the grants 525/040017 and 301/03/H005 from the Grant agency of Czech republic and MSM0021622415 from Ministry of Education of the Czech Republic References: 1) Walterová, D., Ulrychová, J., Válka, I., et al., Acta Univ. Palacki Olomouc Fac. Med. 1995; 139:7-14. 2) Šimánek, V., Vespalec, R., Šedo,A., Ulrichová, J., Vičar, J. 2003. Quaternary Benzo(c)phenanthridine alkaloids - biological activities. In: M.P.Schneider (Ed.), Chemical Probes in Biology. Kluwer Academic Publishers, Netherlands. pp 245-254. 3) Slaninová,I., E.Taborská, H.Bochořáková, and J.Slanina. 2001. Interaction of benzo[c]phenanthridine and protoberberine alkaloids with animal and yeast cells. Cell Biology and Toxicology 17:51-63.
In Czech
Qvarterní benzo[c]phenanthridinové alkaloidy (QBA) jsou skupinou přírodních produktů, které jsou v současnosti diskutovány jako potenciální cytostatika. Vyskytují se v rostlinách čeledí Papaveraceae, Fumariaceae a Rutaceae. Nejznámější a nejlépe prostudované jsou Sanguinarin and chelerytrin, informace o biologických aktivitách minoritních derivátů jsou vzácné. Tato studie se zabývá srovnáním antiproliferační aktivity sanguinarinu (SA), chelerythrinu (CHE) a minoritních derivátů sanguirubine (SR), chelirubine (CHR) and macarpinu (MA) na linii normálních lidských kožních fibroblastů a 3 nádorové buněčné linie (HeLa; A 431; HL-60). Antiproliferační aktivita byla stanovena po 72 hodinové kultivaci pomocí MTT assae, schopnost indukovat apoptózu byla studována pomocí kombinace Anexinu V-FITC a propidium jodidu a hodnocena kvantitastivne průtokovou cytometrií.
Links
| GA525/04/0017, research and development project |
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| GD301/03/H005, research and development project |
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| MSM0021622415, plan (intention) |
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