Instrumentation strategy for investigation of bacteriophage 812
proteome by on-line combination of capillary electrophoresis,
electrochromatography and liquid chromatography with mass
spectrometry

D 2005

Instrumentation strategy for investigation of bacteriophage 812 proteome by on-line combination of capillary electrophoresis, electrochromatography and liquid chromatography with mass spectrometry

KLEPÁRNÍK, Karel, František FORET, Michal SPĚŠNÝ, Jan PREISLER, Zbyněk ZDRÁHAL et. al.

Basic information

Original name

Instrumentation strategy for investigation of bacteriophage 812 proteome by on-line combination of capillary electrophoresis, electrochromatography and liquid chromatography with mass spectrometry

Name in Czech

Instrumentation strategy for investigation of bacteriophage 812 proteome by on-line combination of capillary electrophoresis, electrochromatography and liquid chromatography with mass spectrometry

Authors

KLEPÁRNÍK, Karel (203 Czech Republic, guarantor), František FORET (203 Czech Republic), Michal SPĚŠNÝ (203 Czech Republic), Jan PREISLER (203 Czech Republic), Zbyněk ZDRÁHAL (203 Czech Republic) and Jiří DOŠKAŘ (203 Czech Republic)

Edition

New Orleans, LA USA, 18th International Symposium on MicroScale Bioseparations (MSB), p. 09-89-Tu, 1 pp. 2005

Publisher

California Separation Science Society (CaSSS)

Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

10406 Analytical chemistry

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

RIV identification code

RIV/00216224:14310/05:00013274

Organization unit

Faculty of Science

Keywords in English

BACTERIOPHAGE; PROTEOME; CAPILLARY ELECTROPHORESIS; ELECTROCHROMATOGRAPHY; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY

Abstract

ORIG CZ

V originále

We have developed a new miniaturized instrumentation for combination of the microscale liquid phase separation techniques with mass spectrometry (MS). Its capability will be demonstrated on analyses of proteins prepared from bacteriophage 812. The optimum conditions for growing bacteriophages, preparation of phage proteins and their tryptic digests, and the capillary separations provided a satisfactory amount of peptides for the protein identification by MS with electrospray. The capillary separation columns and nano-spray needles made of narrow bore (ID 5 50 micrometers) silica-fused capillaries were connected by an original laboratory-made interface. The ultimate goal of our research is to contribute to the understanding of the molecular principle of phage therapy. The alarming increase in antibiotic-resistant bacteria has renewed interest in bacteriophage therapy of various human infections. The knowledge of bacteriophages lytic enzymes and cell growth inhibitors can potentially lead to the development of low-molecular species with a specific therapeutic activity.

In Czech

neuvedeno

Links

GA203/03/0515, research and development project

Name: Integrovaná analýza genomu a proteomu terapeuticky významných bakteriofágů kombinací elektroforézy a hmotnostní spektrometrie

Investor: Czech Science Foundation, Integrated genome and proteome analysis of therapeutically important bacteriophages by combination of electrophoresis and mass spectrometry

Citovat

KLEPÁRNÍK, Karel, František FORET, Michal SPĚŠNÝ, Jan PREISLER, Zbyněk ZDRÁHAL and Jiří DOŠKAŘ. Instrumentation strategy for investigation of bacteriophage 812 proteome by on-line combination of capillary electrophoresis, electrochromatography and liquid chromatography with mass spectrometry. In 18th International Symposium on MicroScale Bioseparations (MSB). New Orleans, LA USA: California Separation Science Society (CaSSS), 2005, p. 09-89-Tu, 1 pp.

@inproceedings{627884,
   author = {Klepárník, Karel and Foret, František and Spěšný, Michal and Preisler, Jan and Zdráhal, Zbyněk and Doškař, Jiří},
   address = {New Orleans, LA USA},
   booktitle = {18th International Symposium on MicroScale Bioseparations (MSB)},
   keywords = {BACTERIOPHAGE; PROTEOME; CAPILLARY ELECTROPHORESIS; ELECTROCHROMATOGRAPHY; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY},
   language = {eng},
   location = {New Orleans, LA USA},
   pages = {09-89-Tu},
   publisher = {California Separation Science Society (CaSSS)},
   title = {Instrumentation strategy for investigation of bacteriophage 812 proteome by on-line combination of capillary electrophoresis, electrochromatography and liquid chromatography with mass spectrometry},
   url = {http://www.casss.org/meetings/2005msb/default.htm},
   year = {2005}
}

TY  - JOUR
ID  - 627884
AU  - Klepárník, Karel - Foret, František - Spěšný, Michal - Preisler, Jan - Zdráhal, Zbyněk - Doškař, Jiří
PY  - 2005
TI  - Instrumentation strategy for investigation of bacteriophage 812 proteome by on-line combination of capillary electrophoresis, electrochromatography and liquid chromatography with mass spectrometry
PB  - California Separation Science Society (CaSSS)
CY  - New Orleans, LA USA
KW  - BACTERIOPHAGE
KW  - PROTEOME
KW  - CAPILLARY ELECTROPHORESIS
KW  - ELECTROCHROMATOGRAPHY
KW  - LIQUID CHROMATOGRAPHY
KW  - MASS SPECTROMETRY
UR  - http://www.casss.org/meetings/2005msb/default.htm
N2  - We have developed a new miniaturized instrumentation for combination of the microscale liquid phase separation techniques with mass spectrometry (MS). Its capability will be demonstrated on analyses of proteins prepared from bacteriophage 812. The optimum conditions for growing bacteriophages, preparation of phage proteins and their tryptic digests, and the capillary separations provided a satisfactory amount of peptides for the protein identification by MS with electrospray. The capillary separation columns and nano-spray needles made of narrow bore (ID 5 50 micrometers) silica-fused capillaries were connected by an original laboratory-made interface. The ultimate goal of our research is to contribute to the understanding of the molecular principle of phage therapy. The alarming increase in antibiotic-resistant bacteria has renewed interest in bacteriophage therapy of various human infections. The knowledge of bacteriophages lytic enzymes and cell growth inhibitors can potentially lead to the development of low-molecular species with a specific therapeutic activity.
ER  -

KLEPÁRNÍK, Karel, František FORET, Michal SPĚŠNÝ, Jan PREISLER, Zbyněk ZDRÁHAL and Jiří DOŠKAŘ. Instrumentation strategy for investigation of bacteriophage 812 proteome by on-line combination of capillary electrophoresis, electrochromatography and liquid chromatography with mass spectrometry. In \textit{18th International Symposium on MicroScale Bioseparations (MSB)}. New Orleans, LA USA: California Separation Science Society (CaSSS), 2005, p.~09-89-Tu, 1 pp.

Detailed Information on Publication Record