The aim of this paper is to observe the caries development in humans from Znojmo-Hradiště, CZ. This investigation was performed on the osteological material (in possession of the Department of Anthropology, Faculty of Science, Masaryk University Brno), in deliberately selected probes, having fulfilled the following requirements: 1. according to the time period the skulls came from of archaeologically determinable time period; 2. according to the sex the skulls were divided into males and females; 3. according to the age only group of adultus and maturus were selected; and 4. according to the dental type. The aim of this paper is to find out if there is a difference in caries incidence between both examined burial grounds from Znojmo-Hradiště. According to the time period were values of caries intensity and frequency in Znojmo-Hradišt lower by medieval population. According to the sex medieval women had slightly more decayed teeth than men, while in modern time period it was reversed. According to the age grow caries intensity and frequency with the age is higher by modern times population. According to the dental types were the most decaying teeth first and second molars in both samples from Znojmo-Hradiště. There were more decayed teeth from maxillas and from the right side by both followed samples.