Detailed Information on Publication Record
2005
Imaging of mitochondrial apoptogenic proteins released during apoptosis in living and fixed cells.
VAŘECHA, Miroslav, Jana AMRICHOVÁ, Vladan ONDŘEJ, Emilie LUKÁŠOVÁ, Vladimír ULMAN et. al.Basic information
Original name
Imaging of mitochondrial apoptogenic proteins released during apoptosis in living and fixed cells.
Name in Czech
Sledování mitochondriálních apoptotických proteinů uvolněných během apoptózy v živých a fixovaných buňkách.
Authors
VAŘECHA, Miroslav (203 Czech Republic, guarantor), Jana AMRICHOVÁ (203 Czech Republic), Vladan ONDŘEJ (203 Czech Republic), Emilie LUKÁŠOVÁ (203 Czech Republic), Vladimír ULMAN (203 Czech Republic), Petr MATULA (203 Czech Republic), Michal KOZUBEK (203 Czech Republic) and Stanislav KOZUBEK (203 Czech Republic)
Edition
Book of Abstracts for 13th Euroconference on Apoptosis, 2005
Other information
Language
English
Type of outcome
Konferenční abstrakt
Field of Study
Genetics and molecular biology
Country of publisher
Belgium
Confidentiality degree
není předmětem státního či obchodního tajemství
RIV identification code
RIV/00216224:14330/05:00013294
Organization unit
Faculty of Informatics
ISBN
963-219-273-7
Keywords in English
living cells; GFP; fluorescent protein; FRET; fluorescent microscopy
Tags
International impact, Reviewed
Změněno: 19/10/2010 11:15, Mgr. Miroslav Vařecha, Ph.D.
V originále
Our project is focused on study of mitochondrial apoptogenic proteins released during apoptosis and associated with caspase-independent nuclear chromatin condensation and degradation. We introduce our automated 2D/3D/4D/FRET high-resolution image data acquisition and analysis system developed to visualize and quantify fluorescence signals in fixed and living cells. For our studies, we utilize the fluorescent proteins and various low-toxicity cell permeable fluorescent probes that make it possible to conduct the non-invasive quantitative visualization in living cells. In our experiments, cells are stably or transiently transfected or cotransfected by lipofection with DNA plasmids coding fusion proteins of mitochondrial apoptogenic proteins (cytochrom c, AIF, amid, and endonuclease g) with one of fluorescence proteins (EGFP, EYFP, or t-HcRed).
In Czech
Sledování mitochondriálních apoptotických proteinů uvolněných během apoptózy v živých a fixovaných buňkách.
Links
| GA202/04/0907, research and development project |
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| GP204/03/D031, research and development project |
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| GP204/05/P090, research and development project |
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| MSM0021622419, plan (intention) |
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