NOVÁKOVÁ, Dana, Ivo SEDLÁČEK, Roman PANTŮČEK, Vlastimil ŠTĚTINA, Pavel ŠVEC and Petr PETRÁŠ. Staphylococcus equorum and Staphylococcus succinus isolated from human clinical specimens. Journal of Medical Microbiology. Reading, UK: Society for General Microbiology, 2006, vol. 55, No 5, p. 523-528. ISSN 0022-2615.
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Basic information
Original name Staphylococcus equorum and Staphylococcus succinus isolated from human clinical specimens
Name in Czech Charakterizace kmenů Staphylococcus equorum a Staphylococcus succinus izolovaných z klinického materiálu humánního původu
Authors NOVÁKOVÁ, Dana (203 Czech Republic, belonging to the institution), Ivo SEDLÁČEK (203 Czech Republic, guarantor, belonging to the institution), Roman PANTŮČEK (203 Czech Republic, belonging to the institution), Vlastimil ŠTĚTINA (203 Czech Republic), Pavel ŠVEC (203 Czech Republic, belonging to the institution) and Petr PETRÁŠ (203 Czech Republic).
Edition Journal of Medical Microbiology, Reading, UK, Society for General Microbiology, 2006, 0022-2615.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10600 1.6 Biological sciences
Country of publisher United Kingdom of Great Britain and Northern Ireland
Confidentiality degree is not subject to a state or trade secret
WWW [Abstract, Full text]
Impact factor Impact factor: 2.180
RIV identification code RIV/00216224:14310/06:00015634
Organization unit Faculty of Science
UT WoS 000237150200008
Keywords in English Staphylococcus equorum; Staphylococcus succinus; identification; ribotyping; human clinical material
Tags Human clinical material, identification, ribotyping, Staphylococcus equorum, Staphylococcus succinus
Tags International impact, Reviewed
Changed by Changed by: Ing. Monika Kuchlei Sieberová, učo 137219. Changed: 21/12/2017 15:28.
Abstract
A polyphasic identification approach was applied to a group of 11 novobiocin-resistant staphylococci isolated from human clinical materials. Phenotypic characteristics obtained by both commercial and conventional tests assigned eight strains as Staphylococcus xylosus and three strains as ambiguous S. xylosus/ Staphylococcus equorum. In contrast to biotyping, ribotyping with EcoRI and HindIII and wholle-cell protein fingerprinting assigned six analysed strains as S. equorum, and five strains as Staphylococcus succinus. Confirmation of the identification was done by partial 16S rRNA gene sequencing and S. equorum isolates were verified by a previously described PCR assay targeting the sodA gene. From the data it has been implied that ribotyping and whole-cell protein analysis can be used to differentiate between the biochemically almost indistinguishable species S.xylosus, S. equorum or S. succinus. The present study confirms what is believed to be first occurrence of S. equorum strains in the relevant human clinical material in the Czech Republic and describes what is believed to be first-ever isolation of S. succinus from human clinical material.
Abstract (in Czech)
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Links
GA301/02/1505, research and development projectName: Molekulární diagnostika, epidemiologie a klasifikace klinicky významných grampozitivních koků
Investor: Czech Science Foundation, Molecular diagnostic, epidemiology and classification of pathogenic Gram positive cocci
GP204/02/D099, research and development projectName: Knihovna ribotypizačních profilů typových kultur bakterií
Investor: Czech Science Foundation, Database of the ribotype profiles of bacterial type strains
MSM0021622416, plan (intention)Name: Diverzita biotických společenstev a populací: kauzální analýza variability v prostoru a čase
Investor: Ministry of Education, Youth and Sports of the CR, Diversity of Biotic Communities and Populations: Causal Analysis of variation in space and time
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