J 2006

Staphylococcus equorum and Staphylococcus succinus isolated from human clinical specimens

NOVÁKOVÁ, Dana, Ivo SEDLÁČEK, Roman PANTŮČEK, Vlastimil ŠTĚTINA, Pavel ŠVEC et. al.

Basic information

Original name

Staphylococcus equorum and Staphylococcus succinus isolated from human clinical specimens

Name in Czech

Charakterizace kmenů Staphylococcus equorum a Staphylococcus succinus izolovaných z klinického materiálu humánního původu

Authors

NOVÁKOVÁ, Dana (203 Czech Republic, belonging to the institution), Ivo SEDLÁČEK (203 Czech Republic, guarantor, belonging to the institution), Roman PANTŮČEK (203 Czech Republic, belonging to the institution), Vlastimil ŠTĚTINA (203 Czech Republic), Pavel ŠVEC (203 Czech Republic, belonging to the institution) and Petr PETRÁŠ (203 Czech Republic)

Edition

Journal of Medical Microbiology, Reading, UK, Society for General Microbiology, 2006, 0022-2615

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 2.180

RIV identification code

RIV/00216224:14310/06:00015634

Organization unit

Faculty of Science

UT WoS

000237150200008

Keywords in English

Staphylococcus equorum; Staphylococcus succinus; identification; ribotyping; human clinical material

Tags

International impact, Reviewed
Změněno: 21/12/2017 15:28, Ing. Monika Kuchlei Sieberová

Abstract

V originále

A polyphasic identification approach was applied to a group of 11 novobiocin-resistant staphylococci isolated from human clinical materials. Phenotypic characteristics obtained by both commercial and conventional tests assigned eight strains as Staphylococcus xylosus and three strains as ambiguous S. xylosus/ Staphylococcus equorum. In contrast to biotyping, ribotyping with EcoRI and HindIII and wholle-cell protein fingerprinting assigned six analysed strains as S. equorum, and five strains as Staphylococcus succinus. Confirmation of the identification was done by partial 16S rRNA gene sequencing and S. equorum isolates were verified by a previously described PCR assay targeting the sodA gene. From the data it has been implied that ribotyping and whole-cell protein analysis can be used to differentiate between the biochemically almost indistinguishable species S.xylosus, S. equorum or S. succinus. The present study confirms what is believed to be first occurrence of S. equorum strains in the relevant human clinical material in the Czech Republic and describes what is believed to be first-ever isolation of S. succinus from human clinical material.

In Czech

neuvedeno

Links

GA301/02/1505, research and development project
Name: Molekulární diagnostika, epidemiologie a klasifikace klinicky významných grampozitivních koků
Investor: Czech Science Foundation, Molecular diagnostic, epidemiology and classification of pathogenic Gram positive cocci
GP204/02/D099, research and development project
Name: Knihovna ribotypizačních profilů typových kultur bakterií
Investor: Czech Science Foundation, Database of the ribotype profiles of bacterial type strains
MSM0021622416, plan (intention)
Name: Diverzita biotických společenstev a populací: kauzální analýza variability v prostoru a čase
Investor: Ministry of Education, Youth and Sports of the CR, Diversity of Biotic Communities and Populations: Causal Analysis of variation in space and time