Thermal stability and energetics of 15-mer DNA duplex
interstrand crosslinked by trans-diamminedichloroplatinum(II)

J 2005

Thermal stability and energetics of 15-mer DNA duplex interstrand crosslinked by trans-diamminedichloroplatinum(II)

BRABEC, Viktor and Ctirad HOFR

Basic information

Original name

Thermal stability and energetics of 15-mer DNA duplex interstrand crosslinked by trans-diamminedichloroplatinum(II)

Name in Czech

Teplotní stabilita a energetika 15-meru DNA duplexu obsahujícího meziřetězový můstek vytvořený transplatinou

Authors

BRABEC, Viktor and Ctirad HOFR

Edition

BIOPOLYMERS, 111 RIVER ST, HOBOKEN, NJ 07030 USA, JOHN WILEY & SONS INC, 2005, 0006-3525

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10610 Biophysics

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 2.545

Organization unit

Faculty of Science

UT WoS

000227248900006

Keywords in English

DNA; interstrand cross-link; transplatin; calorimetry; van't Hoff enthalpy; circular dichroism

Abstract

V originále

The effect of the location of the interstrand cross-link formed by trans-diamminedichloroplatinum( II) (transplatin) on the thermal stability and energetics of 15-mer DNA duplex has been investigated. The duplex containing single, site-specific cross-link, thermodynamically equivalent model structures (hairpins) and nonmodified duplexes were characterized by differential scanning calorimetry, temperature-dependent uv absorption, and circular dichroism. The results demonstrate that the formation of the interstrand cross-link of transplatin does not affect pronouncedly thermodynamic stability of DNA: the cross-link induces no marked changes not only in enthalpy, but also in reduced (concentration independent) monomolecular transition entropy. These results are consistent with the previous observations that interstrand cross-links of transplatin structurally perturb DNA only to a relatively small extent. On the other hand, constraining the duplex with the interstrand cross-link of transplatin results in a significant increase in thermal stability that is primarily due to entropic effects: the cross-link reduces the molecularity of the oligomer system from bimolecular to monomolecular. Importantly, the position of the interstrand cross-link within the duplex modulates cooperativity of the melting transition of the duplex and consequently its thermal stability.

Citovat

BRABEC, Viktor and Ctirad HOFR. Thermal stability and energetics of 15-mer DNA duplex interstrand crosslinked by trans-diamminedichloroplatinum(II). BIOPOLYMERS. 111 RIVER ST, HOBOKEN, NJ 07030 USA: JOHN WILEY & SONS INC, 2005, vol. 77, No 4, p. 222-229, 9 pp. ISSN 0006-3525.

@article{632127,
   author = {Brabec, Viktor and Hofr, Ctirad},
   article_location = {111 RIVER ST, HOBOKEN, NJ 07030 USA},
   article_number = {4},
   keywords = {DNA; interstrand cross-link; transplatin; calorimetry; van't Hoff enthalpy; circular dichroism},
   language = {eng},
   issn = {0006-3525},
   journal = {BIOPOLYMERS},
   title = {Thermal stability and energetics of 15-mer DNA duplex interstrand crosslinked by trans-diamminedichloroplatinum(II)},
   url = {http://www3.interscience.wiley.com/cgi-bin/fulltext/109873226/PDFSTART},
   volume = {77},
   year = {2005}
}

TY  - JOUR
ID  - 632127
AU  - Brabec, Viktor - Hofr, Ctirad
PY  - 2005
TI  - Thermal stability and energetics of 15-mer DNA duplex interstrand crosslinked by trans-diamminedichloroplatinum(II)
JF  - BIOPOLYMERS
VL  - 77
IS  - 4
SP  - 222-229
EP  - 222-229
PB  - JOHN WILEY & SONS INC
SN  - 00063525
KW  - DNA
KW  - interstrand cross-link
KW  - transplatin
KW  - calorimetry
KW  - van't Hoff enthalpy
KW  - circular dichroism
UR  - http://www3.interscience.wiley.com/cgi-bin/fulltext/109873226/PDFSTART
N2  - The effect of the location of the interstrand cross-link formed by trans-diamminedichloroplatinum( II) (transplatin) on the thermal stability and energetics of 15-mer DNA duplex has been investigated. The duplex containing single, site-specific cross-link, thermodynamically equivalent model structures (hairpins) and nonmodified duplexes were characterized by differential scanning calorimetry, temperature-dependent uv absorption, and circular dichroism. The results demonstrate that the formation of the interstrand cross-link of transplatin does not affect pronouncedly thermodynamic stability of DNA: the cross-link induces no marked changes not only in enthalpy, but also in reduced (concentration independent) monomolecular transition entropy. These results are consistent with the previous observations that interstrand cross-links of transplatin structurally perturb DNA only to a relatively small extent. On the other hand, constraining the duplex with the interstrand cross-link of transplatin results in a significant increase in thermal stability that is primarily due to entropic effects: the cross-link reduces the molecularity of the oligomer system from bimolecular to monomolecular. Importantly, the position of the interstrand cross-link within the duplex modulates cooperativity of the melting transition of the duplex and consequently its thermal stability.
ER  -

BRABEC, Viktor and Ctirad HOFR. Thermal stability and energetics of 15-mer DNA duplex interstrand crosslinked by trans-diamminedichloroplatinum(II). \textit{BIOPOLYMERS}. 111 RIVER ST, HOBOKEN, NJ 07030 USA: JOHN WILEY \&{} SONS INC, 2005, vol.~77, No~4, p.~222-229, 9 pp. ISSN~0006-3525.

Detailed Information on Publication Record