BRABEC, Viktor and Ctirad HOFR. Thermal stability and energetics of 15-mer DNA duplex interstrand crosslinked by trans-diamminedichloroplatinum(II). BIOPOLYMERS. 111 RIVER ST, HOBOKEN, NJ 07030 USA: JOHN WILEY & SONS INC, vol. 77, No 4, p. 222-229, 9 pp. ISSN 0006-3525. 2005.
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Basic information
Original name Thermal stability and energetics of 15-mer DNA duplex interstrand crosslinked by trans-diamminedichloroplatinum(II)
Name in Czech Teplotní stabilita a energetika 15-meru DNA duplexu obsahujícího meziřetězový můstek vytvořený transplatinou
Authors BRABEC, Viktor and Ctirad HOFR.
Edition BIOPOLYMERS, 111 RIVER ST, HOBOKEN, NJ 07030 USA, JOHN WILEY & SONS INC, 2005, 0006-3525.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10610 Biophysics
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
WWW URL
Impact factor Impact factor: 2.545
Organization unit Faculty of Science
UT WoS 000227248900006
Keywords in English DNA; interstrand cross-link; transplatin; calorimetry; van't Hoff enthalpy; circular dichroism
Tags calorimetry, circular dichroism, DNA, interstrand cross-link, transplatin, van't Hoff enthalpy
Changed by Changed by: doc. Mgr. Ctirad Hofr, Ph.D., učo 7807. Changed: 19/10/2011 13:50.
Abstract
The effect of the location of the interstrand cross-link formed by trans-diamminedichloroplatinum( II) (transplatin) on the thermal stability and energetics of 15-mer DNA duplex has been investigated. The duplex containing single, site-specific cross-link, thermodynamically equivalent model structures (hairpins) and nonmodified duplexes were characterized by differential scanning calorimetry, temperature-dependent uv absorption, and circular dichroism. The results demonstrate that the formation of the interstrand cross-link of transplatin does not affect pronouncedly thermodynamic stability of DNA: the cross-link induces no marked changes not only in enthalpy, but also in reduced (concentration independent) monomolecular transition entropy. These results are consistent with the previous observations that interstrand cross-links of transplatin structurally perturb DNA only to a relatively small extent. On the other hand, constraining the duplex with the interstrand cross-link of transplatin results in a significant increase in thermal stability that is primarily due to entropic effects: the cross-link reduces the molecularity of the oligomer system from bimolecular to monomolecular. Importantly, the position of the interstrand cross-link within the duplex modulates cooperativity of the melting transition of the duplex and consequently its thermal stability.
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