Detailed Information on Publication Record
2006
Ergosterol treatment leads to the expression of a specific set of defence-related genes in tobacco
LOCHMAN, Jan and Vladimír MIKEŠBasic information
Original name
Ergosterol treatment leads to the expression of a specific set of defence-related genes in tobacco
Name in Czech
Ergosterol vyvolává expresi specifických obranných genů u tabáku
Authors
LOCHMAN, Jan (203 Czech Republic) and Vladimír MIKEŠ (203 Czech Republic, guarantor)
Edition
Plant Molecular Biology, UK, Springer, 2006, 0167-4412
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
Genetics and molecular biology
Country of publisher
United Kingdom of Great Britain and Northern Ireland
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 3.577
RIV identification code
RIV/00216224:14310/06:00015653
Organization unit
Faculty of Science
UT WoS
000240399300004
Keywords in English
defence genes; Nicotiana tabacum; ergosterol; elicitor; salicylic acid; plant defence
Změněno: 18/2/2007 10:57, prof. RNDr. Vladimír Mikeš, CSc.
V originále
Ergosterol is the main sterol of most fungi. Production of reactive oxygen species after the treatment of tobacco and tomato cells by nano-molar concentrations of ergosterol was previously observed as well as the activation of some stress activated mitogen-activated protein kinases on alfalfa cells. In this paper, the expression of some defence-related genes after the ergosterol treatment of tobacco Nicotiana tabacum plants is reported. The gene expression of pathogenesis related proteins of families PR1, PR3, PR5 and proteinase inhibitors of class I and II together with enzymes participating in the defence response, such as phenylalanine ammonia lyase and sesquiterpene cyclase, were monitored by RT-qPCR. In addition, the concentrations of salicylic acid, an important signalling molecule, increased in time due to the enzyme activation. On the other hand, ergosterol did not provoke tissue necrosis and the possible cross-talk between the signalling pathways of salicylate and jasmonate was observed. Collected data shows that ergosterol is able to activate the expression of a number of defence genes and could increase resistance against pathogens.
In Czech
Erosterol vyvolává expresi obranných genů u tabáku: PR1, PR3, PR5,PAL a kys. salicylové.
Links
| GA522/06/0156, research and development project |
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| MSM0021622413, plan (intention) |
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