| JEŽEK, Jan, Miroslav VAŘECHA, Jana AMRICHOVÁ, Michal KOZUBEK, Tomáš ČIŽMÁR and Pavel ZEMÁNEK. Optical tracking of microobjects within living cells. In Proceedings of SPIE. 6180th ed. Bellingham, USA: International Society for Optical Engineering, 2006, p. 618025-1, 6 pp. ISBN 0-8194-6236-5. |
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@inproceedings{634172,
author = {Ježek, Jan and Vařecha, Miroslav and Amrichová, Jana and Kozubek, Michal and Čižmár, Tomáš and Zemánek, Pavel},
address = {Bellingham, USA},
booktitle = {Proceedings of SPIE},
edition = {6180},
keywords = {living cells; liposomes; optical microscopy; visualisation; optical tracking},
language = {eng},
location = {Bellingham, USA},
isbn = {0-8194-6236-5},
pages = {618025-1},
publisher = {International Society for Optical Engineering},
title = {Optical tracking of microobjects within living cells},
year = {2006}
}
TY - JOUR
ID - 634172
AU - Ježek, Jan - Vařecha, Miroslav - Amrichová, Jana - Kozubek, Michal - Čižmár, Tomáš - Zemánek, Pavel
PY - 2006
TI - Optical tracking of microobjects within living cells
PB - International Society for Optical Engineering
CY - Bellingham, USA
SN - 0819462365
KW - living cells
KW - liposomes
KW - optical microscopy
KW - visualisation
KW - optical tracking
N2 - Tracing of foreign objects inside living cells is very exciting way how to study interior of living objects in nondestructive way. We imported fluorescent submicron particles into the living cells using liposomes as carriers to study the local mechanical heterogeneity of the cell cytoplasm. Thermal motion of these probes within the cell is tracked using fluorescent video-microscopy. The time-records of the probe positions reveal their trajectories and accessible space to the probes inside the cytoplasm of living cells. Further analyses of the thermal motion of the probes can reveal the mechanism of sub-cellular transport and properties of the cytoplasm in vivo.
ER -
JEŽEK, Jan, Miroslav VAŘECHA, Jana AMRICHOVÁ, Michal KOZUBEK, Tomáš ČIŽMÁR and Pavel ZEMÁNEK. Optical tracking of microobjects within living cells. In \textit{Proceedings of SPIE}. 6180th ed. Bellingham, USA: International Society for Optical Engineering, 2006, p.~618025-1, 6 pp. ISBN~0-8194-6236-5.
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