Detailed Information on Publication Record
2006
Optical tracking of microobjects within living cells
JEŽEK, Jan, Miroslav VAŘECHA, Jana AMRICHOVÁ, Michal KOZUBEK, Tomáš ČIŽMÁR et. al.Basic information
Original name
Optical tracking of microobjects within living cells
Name in Czech
Sledování trajektorie mikroobjektů uvnitř živých buněk
Authors
JEŽEK, Jan (203 Czech Republic), Miroslav VAŘECHA (203 Czech Republic, guarantor), Jana AMRICHOVÁ (203 Czech Republic), Michal KOZUBEK (203 Czech Republic), Tomáš ČIŽMÁR (203 Czech Republic) and Pavel ZEMÁNEK (203 Czech Republic)
Edition
6180. vyd. Bellingham, USA, Proceedings of SPIE, p. 618025-1, 6 pp. 2006
Publisher
International Society for Optical Engineering
Other information
Language
English
Type of outcome
Stať ve sborníku
Field of Study
10610 Biophysics
Country of publisher
United States of America
Confidentiality degree
není předmětem státního či obchodního tajemství
RIV identification code
RIV/00216224:14330/06:00015327
Organization unit
Faculty of Informatics
ISBN
0-8194-6236-5
UT WoS
000236543600077
Keywords in English
living cells; liposomes; optical microscopy; visualisation; optical tracking
Tags
International impact, Reviewed
Změněno: 11/6/2009 10:42, Mgr. Miroslav Vařecha, Ph.D.
V originále
Tracing of foreign objects inside living cells is very exciting way how to study interior of living objects in nondestructive way. We imported fluorescent submicron particles into the living cells using liposomes as carriers to study the local mechanical heterogeneity of the cell cytoplasm. Thermal motion of these probes within the cell is tracked using fluorescent video-microscopy. The time-records of the probe positions reveal their trajectories and accessible space to the probes inside the cytoplasm of living cells. Further analyses of the thermal motion of the probes can reveal the mechanism of sub-cellular transport and properties of the cytoplasm in vivo.
In Czech
Sledování cizího objektu uvnitř živých buněk, bez narušení struktury a životaschopnosti buněk. Pomocí lipozomů se do buněk přirozeněn přenesly fluorescenční mikrokuličky, u kterých jsme pak sledovali samovolný tepelný pohyb těchto objektů s pomocí video-mikroskopie.
Links
| IAA1065203, research and development project |
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