Detailed Information on Publication Record
2006
Development of a combined setup for simultaneous detection of total and glycated haemoglobin content in blood samples
PŘIBYL, Jan and Petr SKLÁDALBasic information
Original name
Development of a combined setup for simultaneous detection of total and glycated haemoglobin content in blood samples
Name in Czech
Vývoj kombinovaného systému pro současné stanovení celkového a glykovaného hemoglobinu ve vzorcích krve
Authors
PŘIBYL, Jan (203 Czech Republic) and Petr SKLÁDAL (203 Czech Republic, guarantor)
Edition
Biosensors & Bioelectronics, Oxford, Elsevier Advanced Technology, 2006, 0956-5663
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10600 1.6 Biological sciences
Country of publisher
United Kingdom of Great Britain and Northern Ireland
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 4.132
RIV identification code
RIV/00216224:14310/06:00016801
Organization unit
Faculty of Science
UT WoS
000236756100015
Keywords in English
Biosensor; Piezoelectric sensor; Quartz crystal microbalance; Flow injection analysis
Tags
International impact, Reviewed
Změněno: 16/6/2007 22:32, prof. RNDr. Petr Skládal, CSc.
V originále
An original setup for analysis of glycated haemoglobin (HbA,) in blood is reported. The construction employed a combination of the piezoelectric biosensor for glycated haemoglobin and the flow-through photometric sensor for total haemoglobin (Hb). The modification of gold electrodes with 3-aminophenylboronic acid (APBA) as a specific ligand was studied; the chemisorbed conjugate of APBA with a long-chain thiocompound provided the best affinity for Hb(A1c). The effect of various operating parameters, such as flow rate and instrumental setup, was optimised. The total haemoglobin content was analysed as absorbance of the haemoglobin-cyanide derivative at 540 nm. Only one standard (calibrator) diluted in various ratio was necessary for calibration and 1 mu l of blood was sufficient for analysis. The full range of Hb(A1c) content (4-15%) in blood can be analysed; the working ranges of total and glycated haemoglobin were 50-2000 and 10-90 mu g/ml, respectively. The developed method was successfully evaluated on blood samples collected from diabetics.
In Czech
Byl vyvinut kombinovaný systém na bázi piezoelektrického biosensoru s imobilizovanou kyselinou aminofenylboritou pro specifickou detekci glykvaného hemoglobinu, paralelně se spektrofotometricky stanovuje celkový hemoglobin. Systém je spojen s automatický průtočným analyzátorem FIAlab a je vhodný pro měření vzorků krve.
Links
| MSM0021622413, plan (intention) |
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