Electrochemical immunosensor for detection of Francisella
tularensis

C 2005

Electrochemical immunosensor for detection of Francisella tularensis

SKLÁDAL, Petr, Yveta SYMERSKÁ, Miroslav POHANKA, Bohuslav ŠAFÁŘ, Aleš MACELA et. al.

Basic information

Original name

Electrochemical immunosensor for detection of Francisella tularensis

Name in Czech

Elektrochemický imunosensor pro detekci Francisella tularensis

Authors

SKLÁDAL, Petr (203 Czech Republic, guarantor), Yveta SYMERSKÁ (203 Czech Republic), Miroslav POHANKA (203 Czech Republic), Bohuslav ŠAFÁŘ (203 Czech Republic) and Aleš MACELA (203 Czech Republic)

Edition

1. vyd. Dordrecht, The Netherlands, Defense against bioterror, p. 221-232, 12 pp. NATO Security through science ser. B, 2005

Publisher

Springer

Other information

Language

English

Type of outcome

Kapitola resp. kapitoly v odborné knize

Field of Study

10600 1.6 Biological sciences

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/05:00015144

Organization unit

Faculty of Science

ISBN

1-4020-3385-0

UT WoS

000236493200017

Keywords in English

Biosensor; Biodetection; Biological agent

Abstract

ORIG CZ

V originále

The immunosensor for rapid and sensitive detection of Francisella tularensis (Ft) was developed using screen-printed sensors with multiple (4) Au working electrodes. The sensing zones were modified with a biolayer of anti-Ft polyclonal antibody attached through the covalently immobilized Protein A. The sandwich assay format was employed with anti-Ft IgG-peroxidase conjugate as tracer. For electrochemical measurements, an automated portable detector was developed; it consisted of a digitally controlled potentiostat and four embedded miniperistaltic pumps. The flow-through measurement involved sequential incubations with sample (10 min), tracer (10 min) and substrate mixture (iodide and hydrogen peroxide). The enzymatically-produced iodine was detected electrochemically at - 50 mV. The limit of detection was 100 Ft cells/ml; the response was significantly higher (3x) when using the living cells compared to the heat inactivated ones.

In Czech

Byl vyvinut elektrochemický imunosensor pro rychlou detekci bakterie Francisella tularensis, patřící mezi biologické bojové prostředky.

Links

MSM0021622413, plan (intention)

Name: Proteiny v metabolismu a při interakci organismů s prostředím

Investor: Ministry of Education, Youth and Sports of the CR, Proteins in metabolism and interaction of organisms with the environment

Citovat

SKLÁDAL, Petr, Yveta SYMERSKÁ, Miroslav POHANKA, Bohuslav ŠAFÁŘ and Aleš MACELA. Electrochemical immunosensor for detection of Francisella tularensis. In Defense against bioterror. 1st ed. Dordrecht, The Netherlands: Springer, 2005, p. 221-232. NATO Security through science ser. B. ISBN 1-4020-3385-0.

@inbook{634237,
   author = {Skládal, Petr and Symerská, Yveta and Pohanka, Miroslav and Šafář, Bohuslav and Macela, Aleš},
   address = {Dordrecht, The Netherlands},
   booktitle = {Defense against bioterror},
   edition = {1},
   keywords = {Biosensor; Biodetection; Biological agent},
   language = {eng},
   location = {Dordrecht, The Netherlands},
   isbn = {1-4020-3385-0},
   pages = {221-232},
   publisher = {Springer},
   title = {Electrochemical immunosensor for detection of Francisella tularensis},
   year = {2005}
}

TY  - CHAP
ID  - 634237
AU  - Skládal, Petr - Symerská, Yveta - Pohanka, Miroslav - Šafář, Bohuslav - Macela, Aleš
PY  - 2005
TI  - Electrochemical immunosensor for detection of Francisella tularensis
VL  - NATO Security through science ser. B
PB  - Springer
CY  - Dordrecht, The Netherlands
SN  - 1402033850
KW  - Biosensor
KW  - Biodetection
KW  - Biological agent
N2  - The immunosensor for rapid and sensitive detection of Francisella tularensis (Ft) was developed using screen-printed sensors with multiple (4) Au working electrodes. The sensing zones were modified with a biolayer of anti-Ft polyclonal antibody attached through the covalently immobilized Protein A. The sandwich assay format was employed with anti-Ft IgG-peroxidase conjugate as tracer. For electrochemical measurements, an automated portable detector was developed; it consisted of a digitally controlled potentiostat and four embedded miniperistaltic pumps. The flow-through measurement involved sequential incubations with sample (10 min), tracer (10 min) and substrate mixture (iodide and hydrogen peroxide). The enzymatically-produced iodine was detected electrochemically at - 50 mV. The limit of detection was 100 Ft cells/ml; the response was significantly higher (3x) when using the living cells compared to the heat inactivated ones.
ER  -

SKLÁDAL, Petr, Yveta SYMERSKÁ, Miroslav POHANKA, Bohuslav ŠAFÁŘ and Aleš MACELA. Electrochemical immunosensor for detection of Francisella tularensis. In \textit{Defense against bioterror}. 1st ed. Dordrecht, The Netherlands: Springer, 2005, p.~221-232. NATO Security through science ser. B. ISBN~1-4020-3385-0.

Detailed Information on Publication Record