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@inbook{634237, author = {Skládal, Petr and Symerská, Yveta and Pohanka, Miroslav and Šafář, Bohuslav and Macela, Aleš}, address = {Dordrecht, The Netherlands}, booktitle = {Defense against bioterror}, edition = {1}, keywords = {Biosensor; Biodetection; Biological agent}, language = {eng}, location = {Dordrecht, The Netherlands}, isbn = {1-4020-3385-0}, pages = {221-232}, publisher = {Springer}, title = {Electrochemical immunosensor for detection of Francisella tularensis}, year = {2005} }
TY - CHAP ID - 634237 AU - Skládal, Petr - Symerská, Yveta - Pohanka, Miroslav - Šafář, Bohuslav - Macela, Aleš PY - 2005 TI - Electrochemical immunosensor for detection of Francisella tularensis VL - NATO Security through science ser. B PB - Springer CY - Dordrecht, The Netherlands SN - 1402033850 KW - Biosensor KW - Biodetection KW - Biological agent N2 - The immunosensor for rapid and sensitive detection of Francisella tularensis (Ft) was developed using screen-printed sensors with multiple (4) Au working electrodes. The sensing zones were modified with a biolayer of anti-Ft polyclonal antibody attached through the covalently immobilized Protein A. The sandwich assay format was employed with anti-Ft IgG-peroxidase conjugate as tracer. For electrochemical measurements, an automated portable detector was developed; it consisted of a digitally controlled potentiostat and four embedded miniperistaltic pumps. The flow-through measurement involved sequential incubations with sample (10 min), tracer (10 min) and substrate mixture (iodide and hydrogen peroxide). The enzymatically-produced iodine was detected electrochemically at - 50 mV. The limit of detection was 100 Ft cells/ml; the response was significantly higher (3x) when using the living cells compared to the heat inactivated ones. ER -
SKLÁDAL, Petr, Yveta SYMERSKÁ, Miroslav POHANKA, Bohuslav ŠAFÁŘ and Aleš MACELA. Electrochemical immunosensor for detection of Francisella tularensis. In \textit{Defense against bioterror}. 1st ed. Dordrecht, The Netherlands: Springer, 2005, p.~221-232. NATO Security through science ser. B. ISBN~1-4020-3385-0.
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