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@inbook{634237,
author = {Skládal, Petr and Symerská, Yveta and Pohanka, Miroslav and Šafář, Bohuslav and Macela, Aleš},
address = {Dordrecht, The Netherlands},
booktitle = {Defense against bioterror},
edition = {1},
keywords = {Biosensor; Biodetection; Biological agent},
language = {eng},
location = {Dordrecht, The Netherlands},
isbn = {1-4020-3385-0},
pages = {221-232},
publisher = {Springer},
title = {Electrochemical immunosensor for detection of Francisella tularensis},
year = {2005}
}
TY - CHAP
ID - 634237
AU - Skládal, Petr - Symerská, Yveta - Pohanka, Miroslav - Šafář, Bohuslav - Macela, Aleš
PY - 2005
TI - Electrochemical immunosensor for detection of Francisella tularensis
VL - NATO Security through science ser. B
PB - Springer
CY - Dordrecht, The Netherlands
SN - 1402033850
KW - Biosensor
KW - Biodetection
KW - Biological agent
N2 - The immunosensor for rapid and sensitive detection of Francisella tularensis (Ft) was developed using screen-printed sensors with multiple (4) Au working electrodes. The sensing zones were modified with a biolayer of anti-Ft polyclonal antibody attached through the covalently immobilized Protein A. The sandwich assay format was employed with anti-Ft IgG-peroxidase conjugate as tracer. For electrochemical measurements, an automated portable detector was developed; it consisted of a digitally controlled potentiostat and four embedded miniperistaltic pumps. The flow-through measurement involved sequential incubations with sample (10 min), tracer (10 min) and substrate mixture (iodide and hydrogen peroxide). The enzymatically-produced iodine was detected electrochemically at - 50 mV. The limit of detection was 100 Ft cells/ml; the response was significantly higher (3x) when using the living cells compared to the heat inactivated ones.
ER -
SKLÁDAL, Petr, Yveta SYMERSKÁ, Miroslav POHANKA, Bohuslav ŠAFÁŘ and Aleš MACELA. Electrochemical immunosensor for detection of Francisella tularensis. In \textit{Defense against bioterror}. 1st ed. Dordrecht, The Netherlands: Springer, 2005, p.~221-232. NATO Security through science ser. B. ISBN~1-4020-3385-0.
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