J 2006

Chromatographic analysis of naphthoquinones in plants

BABULA, Petr, Radka MIKELOVÁ, Vojtěch ADAM, David POTĚŠIL, Josef ZEHNÁLEK et. al.

Basic information

Original name

Chromatographic analysis of naphthoquinones in plants

Name in Czech

Chromatografické stanovení naftochinonů v rostlinách

Name (in English)

Chromatographic analysis of naphthoquinones in plants

Authors

BABULA, Petr (203 Czech Republic), Radka MIKELOVÁ (203 Czech Republic, guarantor), Vojtěch ADAM (203 Czech Republic), David POTĚŠIL (203 Czech Republic), Josef ZEHNÁLEK (203 Czech Republic), René KIZEK (203 Czech Republic), Ladislav HAVEL (203 Czech Republic) and Zdenek SLADKÝ (203 Czech Republic)

Edition

Chemické Listy, 2006, 0009-2770

Other information

Language

Czech

Type of outcome

Článek v odborném periodiku

Field of Study

10406 Analytical chemistry

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 0.431

RIV identification code

RIV/00216224:14310/06:00015698

Organization unit

Faculty of Science

UT WoS

000237048100027

Keywords in English

HPLC; In-vitro cultires; Dionaea-muscipula; Plumbagin; Rotundifolia; Juglone; Lawsone
Změněno: 26/5/2006 09:41, Ing. Radka Mikelová, Ph.D.

Abstract

V originále

The aim of the work was to optimize the high performance liquid chromatography coupled with a diode array detector for the determination of naphthoquinones (1,4-naphthoquinone, lawsone, juglone and plumbagin). The optimal conditions were: mobile phase 0.1 mol l(-1) acetic acid - methanol (35: 65 v/v), flow rate 0.8 ml min(1), column and detector temperature 40 C. Detection limits (3 S/N) were 65 ng ml(-1), 50 ng ml(-1), 75 ng ml-1 and 39 ng ml-1 for lawsone, 1,4-naph-tho-qui-none, juglone and plumbagin, respectively. The optimized method was used for the determination of the mentioned naphthoquinones in plants (Dionaea muscipula, Drosera rotundifolia, Drosera spathulata, Drosera capensis and Paulownia tomentosa).

In English

The aim of the work was to optimize the high performance liquid chromatography coupled with a diode array detector for the determination of naphthoquinones (1,4-naphthoquinone, lawsone, juglone and plumbagin). The optimal conditions were: mobile phase 0.1 mol l(-1) acetic acid - methanol (35: 65 v/v), flow rate 0.8 ml min(1), column and detector temperature 40 C. Detection limits (3 S/N) were 65 ng ml(-1), 50 ng ml(-1), 75 ng ml-1 and 39 ng ml-1 for lawsone, 1,4-naph-tho-qui-none, juglone and plumbagin, respectively. The optimized method was used for the determination of the mentioned naphthoquinones in plants (Dionaea muscipula, Drosera rotundifolia, Drosera spathulata, Drosera capensis and Paulownia tomentosa).

Links

GP525/04/P132, research and development project
Name: Studium obranných mechanismů rostlin při stresu způsobeném těžkými kovy