Detailed Information on Publication Record
2006
d with electrochemical deUsing of flow injection analysis coupletection for a determination of metallothionein
HORNA, Aleš, Radka MIKELOVÁ, David POTĚŠIL, Vojtěch ADAM, René KIZEK et. al.Basic information
Original name
d with electrochemical deUsing of flow injection analysis coupletection for a determination of metallothionein
Name in Czech
Použití průtokové injekční analýzy ve spojení s elektrochemickou detekcí pro stanovení metalothioneinu
Authors
HORNA, Aleš (203 Czech Republic), Radka MIKELOVÁ (203 Czech Republic, guarantor), David POTĚŠIL (203 Czech Republic), Vojtěch ADAM (203 Czech Republic) and René KIZEK (203 Czech Republic)
Edition
Orlando, Florida, USA, 57th Pittsburgh Conference on Analytical Chemistry and Applied Spectroscopy, Pittcon 2006, p. 430-22P, 1 pp. 2006
Publisher
The Pittsburgh Conference on Analytical Chemistry and Applied Spectroscopy, Inc.
Other information
Language
English
Type of outcome
Stať ve sborníku
Field of Study
10405 Electrochemistry
Country of publisher
United States of America
Confidentiality degree
není předmětem státního či obchodního tajemství
RIV identification code
RIV/00216224:14310/06:00015699
Organization unit
Faculty of Science
Keywords in English
Flow injection analysis; electrochemical detection; metallothionein; tumor; cancer; metal ions;
Změněno: 18/5/2009 11:48, Ing. Radka Mikelová, Ph.D.
V originále
The metallothionein (MT) family is a class of low molecular weight, intracellular and cysteine-rich proteins presenting high affinity for metal ions. MTs are involved in many pathophysiological processes such as metal ion homeostasis and detoxification, protection against oxidative damage, cell proliferation and apoptosis, chemoresistance and radiotherapy resistance. The MT overexpression is statistical significant at malignant and high grading tumours. Therefore it is necessary to suggest new methods for determination of MT. Electrochemical techniques enables to perform highly sensitive, low cost and easy-to-use determination of many biological active compounds. Here we studied the electrochemical behaviour of metallothionein by both stationary and flow system with electrochemical detection. The stationary electrochemical measurements (square wave voltammetry - SWV) were performed with the AUTOLAB Analyser using a standard cell with three electrodes, where the glassy carbon electrode served as a working electrode. Flow injection analysis with electrochemical detection (FIA-ED) was consisted from a chromatographic pump, a reaction coil and an electrochemical detector (Coulochem III) with glassy carbon electrode as a working electrode. Metallothionein gave signal at potential of 0.8 V using SWV. The SWV detection limit of MT was about units of micrograms per millilitre. In addition we performed the MT analysis using of FIA-ED. It was evident from the hydrodynamic voltammogram that the highest MT signal was obtained at detector potential of 0.8 V. In addition we studied relationship between overexpression of MT and progression of diseases in human tumours. MT overexpression would be a new prognostic marker of progression of tumours disease.
In Czech
Použití průtokové injekční analýzy ve spojení s elektrochemickou detekcí pro stanovení metalothioneinu
Links
GP525/04/P132, research and development project |
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MSM0021622412, plan (intention) |
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