d with electrochemical deUsing of flow injection analysis
coupletection for a determination of metallothionein

D 2006

d with electrochemical deUsing of flow injection analysis coupletection for a determination of metallothionein

HORNA, Aleš, Radka MIKELOVÁ, David POTĚŠIL, Vojtěch ADAM, René KIZEK et. al.

Basic information

Original name

d with electrochemical deUsing of flow injection analysis coupletection for a determination of metallothionein

Name in Czech

Použití průtokové injekční analýzy ve spojení s elektrochemickou detekcí pro stanovení metalothioneinu

Authors

HORNA, Aleš (203 Czech Republic), Radka MIKELOVÁ (203 Czech Republic, guarantor), David POTĚŠIL (203 Czech Republic), Vojtěch ADAM (203 Czech Republic) and René KIZEK (203 Czech Republic)

Edition

Orlando, Florida, USA, 57th Pittsburgh Conference on Analytical Chemistry and Applied Spectroscopy, Pittcon 2006, p. 430-22P, 1 pp. 2006

Publisher

The Pittsburgh Conference on Analytical Chemistry and Applied Spectroscopy, Inc.

Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

10405 Electrochemistry

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/06:00015699

Organization unit

Faculty of Science

Keywords in English

Flow injection analysis; electrochemical detection; metallothionein; tumor; cancer; metal ions;

Abstract

ORIG CZ

V originále

The metallothionein (MT) family is a class of low molecular weight, intracellular and cysteine-rich proteins presenting high affinity for metal ions. MTs are involved in many pathophysiological processes such as metal ion homeostasis and detoxification, protection against oxidative damage, cell proliferation and apoptosis, chemoresistance and radiotherapy resistance. The MT overexpression is statistical significant at malignant and high grading tumours. Therefore it is necessary to suggest new methods for determination of MT. Electrochemical techniques enables to perform highly sensitive, low cost and easy-to-use determination of many biological active compounds. Here we studied the electrochemical behaviour of metallothionein by both stationary and flow system with electrochemical detection. The stationary electrochemical measurements (square wave voltammetry - SWV) were performed with the AUTOLAB Analyser using a standard cell with three electrodes, where the glassy carbon electrode served as a working electrode. Flow injection analysis with electrochemical detection (FIA-ED) was consisted from a chromatographic pump, a reaction coil and an electrochemical detector (Coulochem III) with glassy carbon electrode as a working electrode. Metallothionein gave signal at potential of 0.8 V using SWV. The SWV detection limit of MT was about units of micrograms per millilitre. In addition we performed the MT analysis using of FIA-ED. It was evident from the hydrodynamic voltammogram that the highest MT signal was obtained at detector potential of 0.8 V. In addition we studied relationship between overexpression of MT and progression of diseases in human tumours. MT overexpression would be a new prognostic marker of progression of tumours disease.

In Czech

Použití průtokové injekční analýzy ve spojení s elektrochemickou detekcí pro stanovení metalothioneinu

Links

GP525/04/P132, research and development project

Name: Studium obranných mechanismů rostlin při stresu způsobeném těžkými kovy

MSM0021622412, plan (intention)

Name: Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální, regionální a lokální úrovni (INCHEMBIOL) (Acronym: INCHEMBIOL)

Investor: Ministry of Education, Youth and Sports of the CR, Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL)

Citovat

HORNA, Aleš, Radka MIKELOVÁ, David POTĚŠIL, Vojtěch ADAM and René KIZEK. d with electrochemical deUsing of flow injection analysis coupletection for a determination of metallothionein (Using of flow injection analysis coupled with electrochemical detection for a determination of metallothionein). In 57th Pittsburgh Conference on Analytical Chemistry and Applied Spectroscopy, Pittcon 2006. Orlando, Florida, USA: The Pittsburgh Conference on Analytical Chemistry and Applied Spectroscopy, Inc., 2006, p. 430-22P, 1 pp.

@inproceedings{636133,
   author = {Horna, Aleš and Mikelová, Radka and Potěšil, David and Adam, Vojtěch and Kizek, René},
   address = {Orlando, Florida, USA},
   booktitle = {57th Pittsburgh Conference on Analytical Chemistry and Applied Spectroscopy, Pittcon 2006},
   keywords = {Flow injection analysis; electrochemical detection; metallothionein; tumor; cancer; metal ions;},
   language = {eng},
   location = {Orlando, Florida, USA},
   pages = {430-22P},
   publisher = {The Pittsburgh Conference on Analytical Chemistry and Applied Spectroscopy, Inc.},
   title = {d with electrochemical deUsing of flow injection analysis coupletection for a determination of metallothionein},
   year = {2006}
}

TY  - JOUR
ID  - 636133
AU  - Horna, Aleš - Mikelová, Radka - Potěšil, David - Adam, Vojtěch - Kizek, René
PY  - 2006
TI  - d with electrochemical deUsing of flow injection analysis coupletection for a determination of metallothionein
PB  - The Pittsburgh Conference on Analytical Chemistry and Applied Spectroscopy, Inc.
CY  - Orlando, Florida, USA
KW  - Flow injection analysis
KW  - electrochemical detection
KW  - metallothionein
KW  - tumor
KW  - cancer
KW  - metal ions;
N2  - The metallothionein (MT) family is a class of low molecular weight, intracellular and cysteine-rich proteins presenting high affinity for metal ions. MTs are involved in many pathophysiological processes such as metal ion homeostasis and detoxification, protection against oxidative damage, cell proliferation and apoptosis, chemoresistance and radiotherapy resistance. The MT overexpression is statistical significant at malignant and high grading tumours. Therefore it is necessary to suggest new methods for determination of MT. Electrochemical techniques enables to perform highly sensitive, low cost and easy-to-use determination of many biological active compounds. Here we studied the electrochemical behaviour of metallothionein by both stationary and flow system with electrochemical detection. The stationary electrochemical measurements (square wave voltammetry - SWV) were performed with the AUTOLAB Analyser using a standard cell with three electrodes, where the glassy carbon electrode served as a working electrode. Flow injection analysis with electrochemical detection (FIA-ED) was consisted from a chromatographic pump, a reaction coil and an electrochemical detector (Coulochem III) with glassy carbon electrode as a working electrode. Metallothionein gave signal at potential of 0.8 V using SWV. The SWV detection limit of MT was about units of micrograms per millilitre. In addition we performed the MT analysis using of FIA-ED. It was evident from the hydrodynamic voltammogram that the highest MT signal was obtained at detector potential of 0.8 V. In addition we studied relationship between overexpression of MT and progression of diseases in human tumours. MT overexpression would be a new prognostic marker of progression of tumours disease.
ER  -

HORNA, Aleš, Radka MIKELOVÁ, David POTĚŠIL, Vojtěch ADAM and René KIZEK. d with electrochemical deUsing of flow injection analysis coupletection for a determination of metallothionein (Using of flow injection analysis coupled with electrochemical detection for a determination of metallothionein). In \textit{57th Pittsburgh Conference on Analytical Chemistry and Applied Spectroscopy, Pittcon 2006}. Orlando, Florida, USA: The Pittsburgh Conference on Analytical Chemistry and Applied Spectroscopy, Inc., 2006, p.~430-22P, 1 pp.

Detailed Information on Publication Record