J 2006

Determination of enzymatic activity by capillary electrophoresis (review).

GLATZ, Zdeněk

Basic information

Original name

Determination of enzymatic activity by capillary electrophoresis (review).

Name in Czech

Stanoveni enzymové aktivity pomocí kapilární elektroforézy (review).

Authors

GLATZ, Zdeněk (203 Czech Republic, guarantor)

Edition

Journal of Chromatography B, Amsterdam, The Netherlands, Elsevier, 2006, 1570-0232

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 2.647

RIV identification code

RIV/00216224:14310/06:00015700

Organization unit

Faculty of Science

UT WoS

000240393200004

Keywords in English

capillary electrophoresis; enzymes; enzyme activity

Tags

Tags

International impact, Reviewed
Změněno: 19/5/2009 18:44, prof. RNDr. Zdeněk Glatz, CSc.

Abstract

ORIG CZ

V originále

Enzymes are biological catalysts that play an important role in biochemical reactions necessary for normal growth, maturation and reproduction through whole live world. Their accurate quantitation in biological samples is important in many fields of biochemistry, not only in routine biochemistry and in fundamental research, but also in clinical and pharmacological research and diagnosis. Since the direct measurement of enzymes by masses is impossible, they must be quantified by their catalytic activities. Many different methods have been applied for this purpose so far. Although photometric methods are undoubtedly the most frequently used, separation methods will further gain their position in this field. The article reviews different possibilities for the assay of enzymatic activity by means of capillary electrophoresis (CE). Both the off-line and on-line enzyme assays based on CE are discussed.

In Czech

Tento přehledný článek je věnován možnosti využití kapilární elektroforézy pro stanoveni aktivity enzymů.

Links

GA203/06/0047, research and development project
Name: Využití kapilární elektroforézy pro studium metabolismu léčiv
Investor: Czech Science Foundation, Capillary electrophoresis as a tool for the drug-metabolism studies
LC06023, research and development project
Name: Integrované bioanalytické technologie pro mikroanalýzy a diagnostiku s využitím LIF a hmotnostní spektrometrie
Investor: Ministry of Education, Youth and Sports of the CR
MSM0021622413, plan (intention)
Name: Proteiny v metabolismu a při interakci organismů s prostředím
Investor: Ministry of Education, Youth and Sports of the CR, Proteins in metabolism and interaction of organisms with the environment