GLATZ, Zdeněk. Determination of enzymatic activity by capillary electrophoresis (review). Journal of Chromatography B. Amsterdam, The Netherlands: Elsevier, 2006, vol. 841, 1-2, p. 23-37. ISSN 1570-0232.
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Basic information
Original name Determination of enzymatic activity by capillary electrophoresis (review).
Name in Czech Stanoveni enzymové aktivity pomocí kapilární elektroforézy (review).
Authors GLATZ, Zdeněk (203 Czech Republic, guarantor).
Edition Journal of Chromatography B, Amsterdam, The Netherlands, Elsevier, 2006, 1570-0232.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10600 1.6 Biological sciences
Country of publisher Netherlands
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 2.647
RIV identification code RIV/00216224:14310/06:00015700
Organization unit Faculty of Science
UT WoS 000240393200004
Keywords in English capillary electrophoresis; enzymes; enzyme activity
Tags Capillary electrophoresis, enzyme activity, enzymes
Tags International impact, Reviewed
Changed by Changed by: prof. RNDr. Zdeněk Glatz, CSc., učo 1865. Changed: 19/5/2009 18:44.
Abstract
Enzymes are biological catalysts that play an important role in biochemical reactions necessary for normal growth, maturation and reproduction through whole live world. Their accurate quantitation in biological samples is important in many fields of biochemistry, not only in routine biochemistry and in fundamental research, but also in clinical and pharmacological research and diagnosis. Since the direct measurement of enzymes by masses is impossible, they must be quantified by their catalytic activities. Many different methods have been applied for this purpose so far. Although photometric methods are undoubtedly the most frequently used, separation methods will further gain their position in this field. The article reviews different possibilities for the assay of enzymatic activity by means of capillary electrophoresis (CE). Both the off-line and on-line enzyme assays based on CE are discussed.
Abstract (in Czech)
Tento přehledný článek je věnován možnosti využití kapilární elektroforézy pro stanoveni aktivity enzymů.
Links
GA203/06/0047, research and development projectName: Využití kapilární elektroforézy pro studium metabolismu léčiv
Investor: Czech Science Foundation, Capillary electrophoresis as a tool for the drug-metabolism studies
LC06023, research and development projectName: Integrované bioanalytické technologie pro mikroanalýzy a diagnostiku s využitím LIF a hmotnostní spektrometrie
Investor: Ministry of Education, Youth and Sports of the CR
MSM0021622413, plan (intention)Name: Proteiny v metabolismu a při interakci organismů s prostředím
Investor: Ministry of Education, Youth and Sports of the CR, Proteins in metabolism and interaction of organisms with the environment
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