Detailed Information on Publication Record
2006
Separation of microcystins by capillary electrochromatography in monolithic columns
ZEISBERGEROVÁ, Marta, Vratislav KOŠŤÁL, Markéta ŠRÁMKOVÁ, Pavel BABICA, Luděk BLÁHA et. al.Basic information
Original name
Separation of microcystins by capillary electrochromatography in monolithic columns
Name in Czech
Separace microcystinů pomocí CEC v monolitických kolonách
Authors
ZEISBERGEROVÁ, Marta (203 Czech Republic), Vratislav KOŠŤÁL (203 Czech Republic), Markéta ŠRÁMKOVÁ (203 Czech Republic), Pavel BABICA (203 Czech Republic), Luděk BLÁHA (203 Czech Republic), Zdeněk GLATZ (203 Czech Republic, guarantor) and Vladislav KAHLE (203 Czech Republic)
Edition
Journal of Chromatography B, Amsterdam, The Netherlands, Elsevier, 2006, 1570-0232
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10600 1.6 Biological sciences
Country of publisher
Czech Republic
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 2.647
RIV identification code
RIV/00216224:14310/06:00015701
Organization unit
Faculty of Science
UT WoS
000240393200015
Keywords in English
microcystines; capillary electrochromatography; monolithic columns
Tags
International impact, Reviewed
Změněno: 9/4/2010 10:04, Mgr. Marta Pelcová, Ph.D.
V originále
Contribution on microcystin variant analysis by capillary electrochromatography (CEC) with easily affordable spectrophotometric detection is presented. Two types of reversed-phase capillary columns formed by inorganic or organic polymer monoliths were prepared for this purpose. The analyses were performed isocratically by means of tris(hydroxymethyl) aminomethane (TRIS) buffers of mildly alkaline pH containing 30% (v/v) acetonitrile as the mobile phases. The samples were injected electrokinetically and the analyses were done at the same separation field strength of 500 V/cm. Microcystins were detected at 238 nm. Although both column types differ not only in monolith quality (inorganic versus organic) but also in the length of the aliphatic moiety (C8 versus C12) similar results were achieved. The on-column preconcentration as the encouraging prospect of electrochromatographic technique was also tested. Consequently 5% of column volume was injected in contrast with 0.5% at standard injection scheme resulting in the six times enrichment of the low concentrated cyanobacterial extract at the top of the separation column. From these preliminary results can be seen that the CEC method is fully applicable for rapid microcystins screening.
In Czech
Separace microcystinů byla prováděna pomocí kapilární elektrochromatografie v laboratorně připravených monolitických kolonách.
Links
| GA203/06/1179, research and development project |
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| LC06023, research and development project |
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| MSM0021622413, plan (intention) |
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