Determination of midazolam and its metabolites in rat plasma and perfuse medium by HPLC

JUŘICA, Jan, Josef TOMANDL, Miroslav DOSTÁLEK and Eva HADAŠOVÁ. Determination of midazolam and its metabolites in rat plasma and perfuse medium by HPLC. In CD of the abstracts of the 29th international symposium on capillary chromatography. Belgium: T. Sandra, Research Institute for Chromatography, 2006, p. -, 1 pp.

Basic information

Original name

Determination of midazolam and its metabolites in rat plasma and perfuse medium by HPLC

Name in Czech

Stanovení midazolamu a jeho metabolitů v potkaní plazmě a perfuzním médiu pomocí HPLC

Authors

JUŘICA, Jan, Josef TOMANDL, Miroslav DOSTÁLEK and Eva HADAŠOVÁ

Edition

Belgium, CD of the abstracts of the 29th international symposium on capillary chromatography, p. -, 1 pp. 2006

Publisher

T. Sandra, Research Institute for Chromatography

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Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Italy

Confidentiality degree

není předmětem státního či obchodního tajemství

Organization unit

Faculty of Medicine

Keywords in English

Cytochrome P450; Midazolam; 1 Hydroxymidazolam; 4 Hydroxymidazolam; 1 4 Dihydroxymidazolam; Rat; Perfuzate; Plasma

Tags

1 4 Dihydroxymidazolam, 1 Hydroxymidazolam, 4 Hydroxymidazolam, cytochrome P450, midazolam, Perfuzate, plasma, rat

Tags

International impact

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Abstract

V originále

A new isocratic reverse-phase high-performance liquid chromatography utilising ultraviolet detection for the simultaneous determination of midazolam and its three main hydroxymetabolites in rat plasma and rat liver perfuse medium is described. The present method offers various advantages over the currently used HPLC methods, including speed, sensitivity and reproducibility. Moreover, it may be applicable for modelling and description of possible pharmacological interactions on rat or human isoforms of cytochrome P450.

In Czech

A new isocratic reverse-phase high-performance liquid chromatography utilising ultraviolet detection for the simultaneous determination of midazolam and its three main hydroxymetabolites in rat plasma and rat liver perfuse medium is described. The present method offers various advantages over the currently used HPLC methods, including speed, sensitivity and reproducibility. Moreover, it may be applicable for modelling and description of possible pharmacological interactions on rat or human isoforms of cytochrome P450.

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Links

GA203/06/0047, research and development project	Name: Využití kapilární elektroforézy pro studium metabolismu léčiv Investor: Czech Science Foundation, Capillary electrophoresis as a tool for the drug-metabolism studies
LC06023, research and development project	Name: Integrované bioanalytické technologie pro mikroanalýzy a diagnostiku s využitím LIF a hmotnostní spektrometrie Investor: Ministry of Education, Youth and Sports of the CR
MSM0021622404, plan (intention)	Name: Vnitřní organizace a neurobiologické mechanismy funkčních systémů CNS Investor: Ministry of Education, Youth and Sports of the CR, The internal organisation and neurobiological mechanisms of functional CNS systems under normal and pathological conditions.