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@proceedings{637409,
author = {Hyršl, Pavel and Páčová, Zdenka and Sedláček, Ivo},
booktitle = {Summit Workshop Cost Actions 850 "Biocontrol Symbiosis"},
keywords = {Photorhabdus luminescens spp. kayaii; electrophoresis; phenotypic characteristics; 16S rDNA},
language = {eng},
title = {New isolate of Photorhabdus luminescens ssp. kayaii from the Czech Republic},
year = {2006}
}
TY - CONF
ID - 637409
AU - Hyršl, Pavel - Páčová, Zdenka - Sedláček, Ivo
PY - 2006
TI - New isolate of Photorhabdus luminescens ssp. kayaii from the Czech Republic
KW - Photorhabdus luminescens spp. kayaii
KW - electrophoresis
KW - phenotypic characteristics
KW - 16S rDNA
N2 - We have isolated symbiotic bacteria from the entomopathogenic nematode Heterorhabditis bacteriophora H221 (Pouzdřany). Isolate Photorhabdus sp. CCM 7002 shows clear luminescence which is characteristic for genus Photorhabdus, the only known terrestrial luminescent bacteria. Thus, our isolate was compared with type cultures of Photorhabdus species (P. asymbiotica ssp. asymbiotica, P. asymbiotica ssp. australis, P. luminescens ssp. akhurstii, P. luminescens ssp. kayaii, P. luminescens ssp. laumondii, P. luminescens ssp. luminescens, P. luminescens ssp. thracensis, P. temperata). Phenotypic characteristics were based on identification kits (API 50 CH, API 20 NE, ENTEROtest 24) supplemented with additional conventional tests. The most closely related or similar species to Photorhabdus sp. CCM 7002 based on 98 phenotypic characteristics appears to be P. luminescens. To compare the protein spectrum of studied bacterial species, bacterial proteins were analysed by acrylamide gradient gel electrophoresis (SDS-PAGGE). We found more than 40 protein fractions in the range of 6.5 - 200 kDa. Our data demonstrated significant differences among protein profiles of Photorhabdus taxa. Finally, the 16S rRNA gene sequence comparison of isolate Photorhabdus sp. CCM 7002 and Photorhabdus type strains exhibited 100 % identity with P. luminescens ssp. kayaii, which was described on the basis of two isolates from Turkey, 2004. The strain Photorhabdus sp. CCM 7002 is the first known isolate of this subspecies from the Czech Republic and probably also the second record described globally. This study was supported by grant MSM0021622416.
ER -
HYRŠL, Pavel, Zdenka PÁČOVÁ and Ivo SEDLÁČEK. New isolate of Photorhabdus luminescens ssp. kayaii from the Czech Republic. In \textit{Summit Workshop Cost Actions 850 ''Biocontrol Symbiosis''}. 2006.
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